oligonucleotides used in this study (8)

elife-29118-v2.xml

10.7554/eLife.29118.017

Table 1.Oligonucleotides Used in this Study.

All oligonucleotides used in this study were ordered from IDT with the indicated modifications.

Oligo name	Sequence (5’ to 3’)	Modification(s)
50dupex LAG	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACGCTGCCGAATTCTGGCTTGCTAGGACATTACAGGATCGTTCGGTCTC	None
50duplex LAG dual biotin	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACGCTGCCGAATTCTGGCTTGCTAGGACATTACAGGATCGTTCGGTCTC	Two biotin-modified thymidine residues in BOLD
50duplex LEAD	GAGACCGAACGATCCTGTAATGTCCTAGCAAGCCAGAATTCGGCAGCGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	The six dT residues at the 3’ end are connected by phosphorothioate bonds (*)
50duplex LAG2	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACGCTGCCGAATTCTGGATTGCTAGGACATTACAGGATCGTTCGGTCTC	None
50duplex LEAD2 dual biotin	GAGACCGAACGATCCTGTAATGTCCTAGCAATCCAGAATTCGGCAGCGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	Two biotin-modified thymidine residues in BOLD; the six dT residues at the 3’ end are connected by phosphorothioate bonds (*)
160mer duplex LEAD	AGAGAGTAGAGTTGAGTTGTGATGTGTAGAGTTGTTGTAGAGAAGAGTTGTGAAGTGTTGAGTAGAGAAGAGAAGAGAAGTGTTGTGATGTGTTGAGTAGTGTAGAGTTGAGAAGTAGAGATGTGTTGAGATGAGAAGAGTTGTAGTTGAGTTGAAGTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT*T	The five dT residues at the 3’ end are connected by phosphorothioate bonds (*)
160mer duplex LAG	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCACTTCAACTCAACTACAACTCTTCTCATCTCAACACATCTCTACTTCTCAACTCTACACTACTCAACACATCACAACACTTCTCTTCTCTTCTCTACTCAACACTTCACAACTCTTCTCTACAACAACTCTACACATCACAACTCAACTCTACTCTCT	None
Blocked Fork LEAD	ACCGGAGACCGAACGATCCTGTAATGTCCTAGCAAGCCAGAATTCGGCAGCGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGGAAAGAATGTTGGTGAGGGTTGGGAAGTGGAAGGATGGGCTCGAGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT*T	The five dT residues at the 3’ end are connected by phosphorothioate bonds (*)
Blocked Fork LAG	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACGC TGCCGAATTCTGGCTTGCTAGGACATTACAGGATCGTTCGGTCT*C	Two biotin-modified thymidine residues in BOLD; the five dT residues at the 3’ end are connected by phosphorothioate bonds (*)
Blocked Fork Primer	CCTCTCGAGCCCATCCTTCCACTTCCCAACCCTCACC	None
C2	CCTCTCGAGCCCATCCTTCCACTTCCCAACCCTCACC	None

elife-32057-v1.xml

10.7554/eLife.32057.034

Appendix 1—table 3.Oligonucleotides used in this study.

Oligonucleotide name	Sequence (5’ to 3’)
eYFP-F	CGGCGCGCCTCAGGTTCAGGTTCAGGTATGGTGAGCAAGGGCGAG
eYFP-R	CGCGGCCGCTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGC
GFP-F	CGGCGCGCCTCAGGTTCAGGTTCAGGTATGGCTAGCAAAGGAGAAGAA CTTTTCACTGGAGTTGTCCC
GFP-R	CGCGGCCGCTTATTTGTAGAGCTCATCCATGCCATGTGTAATCCCAGCAGC
SNAP-F	GGGCGCGCCTCAGGTTCAGGTTCAGGTATGGACAAAGACTGCGAAATG AAGCGCAC
SNAP-R	CGAATTCTCATTAACCCAGCCCAGGCTTGCCCAGTCTG
meYFP-F	CTACCAGTCCAAGCTGAGCAAAGAC
meYFP-R	CTCAGGTAGTGGTTGTCG
pOB-ezrA-up-F	TTTACGTACACTATCTGCAGATGCTTCTCCTCCTAATTTATCATT
pOB-ezrA-up-R	ATTCGAGCTCGGTACCCGGGTTTTAAATTAATAAAAAAAACAC CCACAATT
pOB-ezrA-down-F	CACTATAGAATACTCAAGCTTACTCCTTAATTTCCTCATAAATGATGA
pOB-ezrA-down-R	GGATCAACTTTGGGAGAGAGAAACTAGTATGTAGTTATACTTAAA TAATATGAGC
pOB-TetR-F	TAAATTAGGAGGAGAAGCATCTGCAGATAGTGTACGTAAAAAGA
pOB-TetR-R	GTATAACTACATACTAGTTTCTCTCTCCCAAAGTTGATCCC
ftsZ-eyfp-F	ACATGGCCATGTCAGGTTCAG
ftsZ-eyfp-R	GGCGCGCCTTATTTATATAATTC
FGFtsZXhoI-F	CTCGAGATGTTAGAATTTGAACAAGG
FGFtsZEcoRI-R	TTAGAATTCACGTCTTGTTCTTCTTGAA
FGFtsZNheI-F	GTTGCTAGCATGTTAGAATTTGAACAAGG
FGFtsZAscI-R	GTTGGCGCGCCTTATCCCAGACCCGGTTTAC

elife-32358-v1.xml

10.7554/eLife.32358.021

Table 1.Oligonucleotides used in this study

Mouse primers		Oligonucleotide sequences (5’−3’)
Aldh1a1^-/-	Forward	TGAGCAAATCCTCCACAGCCCTGTTC
Aldh1a1^-/-	Reverse	CTGCTAAAGCGCATGCTCCAGACTG
Aldh1a3 flox	Forward	TCTCTGACCAGCTTTCCAACCTTCAG
Aldh1a3 flox	Reverse	CTCAAACCAGCACCACCTCCATATTG
Sox9 flox	Forward	TCAGCAAGACTCTGGGCAAGCTCT
Sox9 flox	Reverse	CTCAAAATCTGAGCCACTCCCTC
Tyr-Cre, Pax6-α-Cre	Forward	CCTGGAAAATGCTTCTGTCCGT
Tyr-Cre, Pax6-α-Cre	Reverse	GTGTCCACATAGTCATTGGCAGAGTG
Sox9-Tg, Kdr-BAC-EGFP	Forward	AGCTGACCCTGAAGTTCATCTG
Sox9-Tg, Kdr-BAC-EGFP	Reverse	GTCGTCCTTGAAGAAGATGGTG
Flt1-BAC-tdsRed	Forward	GCTGCAGGCGCGGAGAAGGGCTCTC
Flt1-BAC-tdsRed	Reverse	CTTCACGTACACCTTGGAGC
IL2 (internal control)	Forward	GCCTAGAAGATGAACTTGGACCTCTG
IL2 (internal control)	Reverse	GTGGAAGGATTCACTTGCACAGTGAC
Human RPE primers
Sox9	Forward	CGTACCCGCACTTGCACAAC
Sox9	Reverse	TCTCGCTCTCGTTCAGAAGTC
Vegfa	Forward	TGCCCGCTGCTGTCTAAT
Vegfa	Reverse	TCTCCGCTCTGAGCAAGG
β-actin	Forward	CCAACCGCGAGAAGATGA
β-actin	Reverse	CCAGAGGCGTACAGGGATAG

elife-32358-v2.xml

10.7554/eLife.32358.021

Table 1.Oligonucleotides used in this study

Mouse primers		Oligonucleotide sequences (5’−3’)
Aldh1a1^-/-	Forward	TGAGCAAATCCTCCACAGCCCTGTTC
Aldh1a1^-/-	Reverse	CTGCTAAAGCGCATGCTCCAGACTG
Aldh1a3 flox	Forward	TCTCTGACCAGCTTTCCAACCTTCAG
Aldh1a3 flox	Reverse	CTCAAACCAGCACCACCTCCATATTG
Sox9 flox	Forward	TCAGCAAGACTCTGGGCAAGCTCT
Sox9 flox	Reverse	CTCAAAATCTGAGCCACTCCCTC
Tyr-Cre, Pax6-α-Cre	Forward	CCTGGAAAATGCTTCTGTCCGT
Tyr-Cre, Pax6-α-Cre	Reverse	GTGTCCACATAGTCATTGGCAGAGTG
Sox9-Tg, Kdr-BAC-EGFP	Forward	AGCTGACCCTGAAGTTCATCTG
Sox9-Tg, Kdr-BAC-EGFP	Reverse	GTCGTCCTTGAAGAAGATGGTG
Flt1-BAC-tdsRed	Forward	GCTGCAGGCGCGGAGAAGGGCTCTC
Flt1-BAC-tdsRed	Reverse	CTTCACGTACACCTTGGAGC
IL2 (internal control)	Forward	GCCTAGAAGATGAACTTGGACCTCTG
IL2 (internal control)	Reverse	GTGGAAGGATTCACTTGCACAGTGAC
Human RPE primers
Sox9	Forward	CGTACCCGCACTTGCACAAC
Sox9	Reverse	TCTCGCTCTCGTTCAGAAGTC
Vegfa	Forward	TGCCCGCTGCTGTCTAAT
Vegfa	Reverse	TCTCCGCTCTGAGCAAGG
β-actin	Forward	CCAACCGCGAGAAGATGA
β-actin	Reverse	CCAGAGGCGTACAGGGATAG

elife-44248-v3.xml

10.7554/eLife.44248.020

Table 1.Oligonucleotides used in this study.

Sequence (5ʹ → 3ʹ)	Description
GATTTTCGCTGTCGCACTCTTCATGGGTGCGTGGATTGAAAT ATTGAcgatagTCAATATTTCAATCCACGCACCCATGAAGAGTGC GACAGCGAAAATC	CRISPR hairpin target^*
GATTTTCGCTGTCGCACTCTTCATGGGTGCGTGGATTGAAAT ATTGAGGTAGGTATTG	Mini-CRISPR array
CAATACCTACCTCAATATTTCAATCCACGCACCC ATGAAGAGTGCGACAGCGAAAATC	RC^†
CGTAGCTGAGGACCACCAGAACAG TTTTGAATTTTTTTT	15-nt 3ʹ overhang prespacer, 4-nt between duplex and PAM^††
CGTAGCTGAGGACCACCAGAACAG TTGAATTTTTTTTTT	2-nt between duplex and PAM
CGTAGCTGAGGACCACCAGAACAG TTTTTTGAATTTTTT	6-nt between duplex and PAM
CGTAGCTGAGGACCACCAGAACAG TTTTTTTTGAATTTT	8-nt between duplex and PAM
CTGTTCTGGTGGTCCTCAGCTACG TTTTGAATTTTTTTT	RC of previous four oligos
GATTTTCGCTGTCGCACTCTTCATGGGTGCGTGGATTGAAATATTGA	CRISPR DNA substrate
TCAATATTTCAATCCACGCACCCATGAAGAGTGCGACAGCGAAAATC	RC
GCGTAGCTGAGGACCACCAGAACAGTTTTGAATTTTTTTTTTTTTTTTTT	25-nt 3ʹ overhang prespacer
GCGTAGCTGAGGACCACCAGAACAG	25 bp duplex
CTGTTCTGGTGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTTGAATTTTTTTTTTTTTTCAGGT CGACAAGCTTG	T-rich ssDNA prespacer
CAAGCTTGTCGACCTGAAAAAAAAAAAAAATTCAAAAAAAAAAAAA GGTCCTCAGCTACGC	RC
CTAGTATGATCATGTCCAACGAATCAATACCTACCTCAATGAACGGAT	48 bp duplex
ATCCGTTCATTGAGGTAGGTATTGATTCGTTGGACATGATCATACTAG	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTTTTTTTGAATTGAATTGAA TTTTTTTTTTTTTTTTTTGACAAGCTTGCGACA	3 PAM sites interspersed in 2-nt
TGTCGCAAGCTTGTCAAAAAAAAAAAAAAAAAATTCAATTCAATTCA AAAAAAAAAAAAAAAAAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTTTTTTTTTGAAGAAGAATTTTT TTTTTTTTTTTTTTTGACAAGCTTGCGACA	3 PAM sites without spacing
TGTCGCAAGCTTGTCAAAAAAAAAAAAAAAAAAAATTCTTCTTCAAA AAAAAAAAAAAAAAAAAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTGAATTTTTTTTTTGAATTTT TTTTTTGAATTTTTTTTTTGACAAGCTTGCGACA	3 PAM sites interspersed with 10-nt
TGTCGCAAGCTTGTCAAAAAAAAAATTCAAAAAAAAAATTCAAAAA AAAAATTCAAAAAAAAAAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTGAATTTTTTTTGAATTTTTTTT GAATTTTTTTTTTTTGACAAGCTTGCGACA	3 PAM sites interspersed with 8-nt
TGTCGCAAGCTTGTCAAAAAAAAAAAATTCAAAAAAAATTCAAAAAAAA TTCAAAAAAAAAAAAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTTTGAATTTTTTGAATTTT TTGAATTTTTTTTTTTTTTGACAAGCTTGCGACA	3 PAM sites interspersed with 6-nt
TGTCGCAAGCTTGTCAAAAAAAAAAAAAATTCAAAAAATTCAAAA AATTCAAAAAAAAAAAAAAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTTTTTGAATTTTGAATTTTGAA TTTTTTTTTTTTTTTTGACAAGCTTGCGACA	3 PAM sites interspersed with 4-nt
TGTCGCAAGCTTGTCAAAAAAAAAAAAAAAATTCAAAATTCAAAA TTCAAAAAAAAAAAAAAAAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTATATATATATATGAATATATATATATATA CAGGTCGACAAGCTTG	AT-rich ssDNA prespacer
CAAGCTTGTCGACCTGTATATATATATATATTCATATATATAT ATAGGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTGGTATTCAACAGAATTTTTTTTTTTTTTCA GGTCGACAAGCTTG	Non-T-rich upstream/T rich downstream ssDNA prespacer
CAAGCTTGTCGACCTGAAAAAAAAAAAAAATTCTGTTGAATACCAAG GTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTTTTTTTTTTTTGAACTCGTATTCAACAG CAGGTCGACAAGCTTG	T-rich upstream/non T-rich downstream ssDNA prespacer
CAAGCTTGTCGACCTGCTGTTGAATACGAGTTCAAAAAAAAAAAAA GGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACCTTGGTATTCAACAGAACTCGTATTC AACAGCAGGTCGACAAGCTTG	Non-T-rich up- and downstream ssDNA prespacer
CAAGCTTGTCGACCTGCTGTTGAATACGAGTTCTGTTGAATACCAA GGTCCTCAGCTACGC	RC
GCGTAGCTGAGGACC	Primer used for ddNTP Sanger sequencing
GCGTAGCTGAGGACCCGTGGCACCGACATGGCATTTTTNNNNGAA TTTTTGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	NNNN on upstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCAGTTTTTNNNGAA TTTTTGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	NNN on upstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCAGGTTTTTNNGAA TTTTTGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	NN on upstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCAGGCTTTTTNGAA TTTTTGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	N on upstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCATTTTTGAANNNN TTTTTGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	NNNN on downstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCATTTTTGAANNNTTTTT CGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	NNN on downstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCATTTTTGAANN TTTTTCAGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	NN on downstream region
GCGTAGCTGAGGACCCGTGGCACCGACATGGCATTTTTGAAN TTTTTCATGCTGGGCGCTAAGGGACAACTCCAGGTCGACAAGCTTG	N on downstream region
CAAGCTTGTCGACCTG	Primer used for primer extension
TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCAAGCTTGTCGACCTG	Primer used for amplification-Forward
GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCGTAGCTGAGGACC	Primer used for amplification-Reverse

^*For CRISPR oligonucleotides, leader is in italics, repeat is in bold, and spacer is in plain uppercase font. For hairpin, the loop region is in lowercase.

^†RC = reverse complement of previous oligonucleotide.

^††For cleavage substrates, PAM sequences are underlined.

elife-45952-v2.xml

10.7554/eLife.45952.014

Table 1.Oligonucleotides used in this study.

Restriction enzyme recognition sequences are capitalised. BsaI overhangs are underlined. Introduced mutations are in bolded capital letters. Additional oligonucleotides used for site-directed mutagenesis are listed in Tables S1 and S2 of Supplementary file 1.

Name	Sequence (5’ to 3’)	Function
F2-pUC19-BsaI	gcgAGATCTgtcgtGAGACCggtgatgacggtgaaaacct	gtsB mutagenesis vector construction
R3-pUC19-MfeI-SpeI	actgcgACTAGTCAATTGattaatgcagctggcacgac	gtsB mutagenesis vector construction
F-800Right	actgcgCAATTGagaccccggaagacatcag	gtsB mutagenesis vector construction
R-800Right	actgcgTCTAGAcattgcgaagttcaagcgta	gtsB mutagenesis vector construction
F2-gtsB-F	actgcgGGTCTCagtcgaaaagtcgcgacctacatgg	Conserved gtsB forward primer
R3-gtsB-R	actgcgGGTCTCctgccggaCaccacggtcggccagctc	Conserved gtsB reverse primer
4845-M13F	GTAAAACGACGGCCAGTTCCGACAGGCTGTAGTCCTT	gtsB sequencing primer
R2-M13R-gtsB	GGAAACAGCTATGACCATGTGGTCCTCAGCTCGGAATA	gtsB sequencing primer
SP1	ACCACACCGAACAGGAAGTC	5’ RACE cDNA synthesis
B-SP2	ACTGCGTCTAGAGACCAAGGTGATACCGATAAACA	5’ RACE gtsB amplification
B-SP3	ACTGCGTCTAGACGAACAAGGCCAGGTTTTT	5’ RACE gtsB amplification
A-SP2	ACTGCGTCTAGATTTCTTGTCGAGCAGGGAGT	5’ RACE gtsA amplification
A-SP3	ACTGCGTCTAGATTCTTCTTTGGCGACGTCTT	5’ RACE gtsA amplification

elife-47365-v2.xml

Table 1.Oligonucleotides used in this study.

Name	Sequence	Reference
agr-a	CGCGGATCCTACATAGCACTGAGTCCAAGG	This study
agr-b	GCCGTTAACTGACTTTATTATCTTTTTTACACCACTCTCCTCACTG	This study
agr-c	CAGTGAGGAGAGTGGTGTAAAAAAGATAATAAAGTCAGTTAACGGC	This study
agr-d	CCGGAATTCCAGTTATTAGCAGGATTTTAGC	This study

elife-56048-v2.xml

Table 3.Oligonucleotides used in this study.

Name	Sequence (5´→3´)
JR163	GCGCCCATGGCTAAGGCATCCATTTCAATAGACGAGAAG
JR164	GCGCGTCGACTTATTCTTTTTCCGTATCCATTTGCTGTA
JR169	GCGCCCATGGATTCAAAAGATCAAACAATGTTTTACAACTTC
JR170	GCGCGTCGACTCATTTCTCACCAATTTCGTTATTTTTCAG
JR197	GCGCGGATCCCAATTATTTCGAATGGTGCGGTGTC
JR198	TCCTTATTCGTCGACCATCTTTCCTCAGTCCCTTCCTG
JR199	GGAAAGATGGTCGACGAATAAGGAATAAATCCTAGTTAGTAGGG
JR200	CGCGCGAATTCCCAAGACTCAACCTCTTTCACTC
JR249	GCGCCTGCAGAAAAAATTATTGTACGCGGTGGAAAAC
JR250	GCGCGGTACCGCGAATAAAGACGCTAAGTTTGTTACATCG
JR253	GCGCTCTAGAAAAGGCATCCATTTCAATAGACGAG
JR254	GCGCGGTACCTCTTTTTCCGTATCCATTTGCTG
JR255	GCGCTCTAGATTCAAAAGATCAAACAATGTTTTACAAC
JR256	GCGCGGTACCTTCTCACCAATTTCGTTATTTTTCAG
JR257	GCGCCTGCAGGGAAAAAATTATTGTACGCGGTGGAAAAC
JR264	GCGCAGATCTGGCAAATACAGCATTGAACTATGTG
JR265	GCGCGGATCCAATCGAAGCACCTCATTCCTTC
JR266	GCGCGGATCCATGAGAATAATGGGTTTAGATGTCGGC
JR267	GCGCGTCGACGCTAGGAATGTAGCAAGGATTTCTTC
SHW815	GATCTATCGATGCATGCCATGGGCTAAATGACCAAGGAATTACCG
SHW816	CGCGTCGGGCGATATCGGATCCTTTCTTCCGCGTTTTGGTAACG
SHW817	CAATCATCATTTTAAAAGCACCTCACTATTTTTCAG
SHW818	TGCTTTTAAAATGATGATTGGTAAGCGATTAAGC
SHW819	GATCTATCGATGCATGCCATGGAGATAGAGGCAGAATAAGACATC
SHW820	CGCGTCGGGCGATATCGGATCCGGTATTTACAACCACTACGTCG
SHW821	CGTTCTTATTTCATGAAGCATCCCTCCCTTTC
SHW822	TGCTTCATGAAATAAGAACGGAGGAAATGTGCTG
SHW830	GCGCGCTCTAGATGGACGATTTACGCAAAGAGCTCAG
SHW831	GCGCGCGGTACCTTAGCTTTTACTTTTTTAGAGGTTGTTTTC
SHW832	GCGCGCTCTAGAAATTCCAACAGTAATTGAACAAACTAGC
SHW833	GCGCGCGGTACCCCTTTTAAGCCAGATTTATTAATGATAATATC
SW77	GTAAAACATTGCTTGATCTTTTGAATCCATGGGTTTCAC
SW78	GATCAAGCAATGTTTTACAACTTCGGCGATGATTC
SW79	GTAAAACATGTCTTGATCTTTTGAATCCATGGGTTTCAC
SW80	GATCAAGACATGTTTTACAACTTCGGCG ATGATTC
SW110	GCGCGCGGATCCATGCATGCAGAATTTAGAACAGATAG
SW111	GCGCGCGTCGACTCATGAAGCATCCCTCCCTTTC
SW112	GCGCGCGGATCCATGATGATTGGTAAGCGATTAAGCG
SW113	GCGCGCGTCGACTTAATTTGGATAAGGGACTGTACCTTC
SW136	CTAAACGAGCTATCATACTTCTAGCATCCTTGTGAC
SW137	GTATGATAGCTCGTTTAGAACGAGATGAAATTATCGAG
SW138	AATTTCATCTGCTTCTAAACGACGTATCATACTTCTAGC
SW139	GTTTAGAAGCAGATGAAATTATCGAGGAACTTGTCAAAG
SW144	CCTTGTGAGCAGGAATATAAGCAGGATCGCCTG
SW145	TATATTCCTGCTCACAAGGATGCTAGAAGTATGATAC
SW146	GTATCATACTTGCAGCATCCTTGTGACGAGGAATATAAG
SW147	GGATGCTGCAAGTATGATACGTCGTTTAGAACGAG
Lmo1503F	GCTATACCATGGATTCAAAAGATCAAACAATGTTTTACAAC
Lmo1503R	CGATATCTCGAGTCATTTCTCACCAATTTCGTTATTTTTCAG
PrkAF	GCTATACCATGGCAATGATGATTGGTAAGCGATTAAGCG
PrkAR	CGATATCTCGAGTCATTTTTTCTTTTTCTTATCTTTTTTCTCCTCAGG
PrpCF	GCTATACCATGGCAATGCATGCAGAATTTAGAACAGATAGAG
PrpCR	CGATATCTCGAGTCATGAAGCATCCCTCCCTTTC