plasmids used in this study (10)

elife-27455-v3.xml

10.7554/eLife.27455.032

Table 3.Plasmids used in this study.

Plasmid	Product	System	Ind	Res	Source
pVS52	CheZ-5G-YFP	pBAD33	ara	cam	Sourjik and Berg, 2002a
pVS149	CheY-5G-mRFP1	pTrc99a	IPTG	amp	Sourjik and Berg, 2002a
pSJAB12	CheZ-5G-YFP/CheY-5G-mRFP1	PTrc99a	IPTG	amp	This work
pSJAB106	CheZ-5G-YFP/CheY-5G-mRFP1^¶	PTrc99a	IPTG	amp	This work
pVS91	CheB^†	pTrc99a	ara	cam	Liberman et al., 2004
pVS97	CheB-D56E^‡	pBAD33	ara	cam	Clausznitzer et al., 2010
pVS112	CheBc^§	pBAD33	ara	cam	V. Sourjik
pSJAB 122	CheBc-GS4G-mVenus	pBAD33	ara	cam	This work
pSJAB 123	CheB(D56E)-GS4G-mVenus	pBAD33	ara	cam	This work
pSJAB 124	CheB-GS4G-mVenus	pBAD33	ara	cam	This work
pZR 1	FliC*	pKG116	NaSal	cam	This work
pPA114 Tsr	Tsr	pPA114	NaSal	cam	Ames et al., 2002

^¶Contains a A206K mutation to enforce monomerity..

^†expresses WT CheB.

^‡carries a point mutation D56E in CheB.

^§expresses only residues 147–349 of CheB, preceded by a start codon (Met).

*expresses sticky FliC filament (Scharf et al., 1998)

elife-30015-v2.xml

10.7554/eLife.30015.023

Table 2.Plasmids used in this study.

Plasmid #	Plasmid name	Description	Bacterial selection	Reference
8	pRS316	pRS316, cen, Ura3	Amp	Sikorski and Hieter, 1989
10	HA-Fzo1 on pRS316	HA-Fzo1 on pRS316, Fzo1 prom, cen, Ura3	Amp	Anton et al. (2013)
14	HA-Fzo1-K464R on pRS316	HA-Fzo1^K464R on pRS316, Fzo1 prom, cen, Ura3	Amp	Anton et al. (2013)
58	YEplac181	YEplac181, 2µ, Leu2	Amp	Gietz and Sugino, 1988
59	Ubp2-Flag on YEplac181	Ubp2-Flag on YEplac181, Adh1 prom, 2µ, Leu2	Amp	Anton et al. (2013)
60	Ubp2-C745S-Flag on YEplac181	Ubp2^C745S-Flag on YEplac181, Adh1 prom, 2µ, Leu2	Amp	Anton et al. (2013)
61	Ubp12-Flag on YEplac181	Ubp2-Flag on YEplac181, Adh1 prom, 2µ, Leu2	Amp	Anton et al. (2013)
62	Ubp12-C372S-Flag on YEplac181	Ubp2^C372S-Flag on YEplac181, Adh1 prom, 2µ, Leu2	Amp	Anton et al. (2013)
63	YEplac195	YEplac195, 2µ, Ura3	Amp	Gietz and Sugino, 1988
65	Ubp12^C372S on YEplac195	Ubp12^C372S (non-tagged) on YEplac195, 2µ, Ura3	Amp	this study
70	mt-GFP on pYX142	mt-GFP on pYX142, cen, Leu2	Amp	Westermann and Neupert, 2000
75	Cdc48 wt on pRS313	Cdc48 wt on pRS313, cen, His3	Amp	Esaki and Ogura (2012)
79	pRS313	pRS313, cen, His3	Amp	Sikorski and Hieter, 1989
150	Cdc48-A547T on pRS313	Cdc48^A547T on pRS313, cen, His3	Amp	this study
341	Ub on pKT10	Ub on pK10, 2µ, Ura3	Amp	Tanaka et al., 1990
342	Ub-K48R on pKT10	Ub^K48R on pK10, 2µ, Ura3	Amp	Tanaka et al., 1990
343	Ub-K63R on pKT10	Ub^K63R on pK10, 2µ, Ura3	Amp	Tanaka et al., 1990
344	Ub-K48R,K63R on pKT10	Ub^K48R,K63R on pK10, 2µ, Ura3	Amp	Tanaka et al., 1990
356	Myc-Ub on pRS426	pCup1-Myc-Ub on pRS426, 2µ, Ura3	Amp	Li et al., 2015
375	pRS426	pRS426, 2µ, Ura3	Amp	Li et al., 2015

elife-30205-v1.xml

10.7554/eLife.30205.018

Table 3.Plasmids used in this study.

Plasmid	Description	Source
pMQ30	7.5 kb mobilizable vector; oriT, sacB, Gm^R.	Shanks et al. (2006)
pAKN69	Contains mini-Tn7(Gm)PA1/04/03::eyfp fusion.	Lambertsen et al. (2004)
pLD2722	GmR, TetR flanked by Flp recombinase target (FRT) sites to resolve out resistance casettes.	this study
pFLP2	Site-specific excision vector with cI857-controlled FLP recombinase encoding sequence, sacB, Ap^R.	Hoang et al. (1998)
pLD1852	∆ccoN1 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1.	this study
pLD1610	∆ccoN2 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1.	this study
pLD1616	∆ccoN3 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1.	this study
pLD1264	∆ccoN4 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1.	this study
pLD1929	∆cco1 cco2 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1.	this study
pLD2791	∆hcn PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1.	this study
pLD1853	Full genomic sequence of ccoN4 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain InvSc1. Verified by sequencing.	this study
pLD1966	∆aa3 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain IncSc1.	this study
pLD1967	∆bo3 PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain IncSc1.	this study
pLD2044	∆cio PCR fragment introduced into pMQ30 by gap repair cloning in yeast strain IncSc1.	this study
pLD2777	PCR-amplified cco1 promoter ligated into pSEK103 using SpeI and XhoI.	this study
pLD2778	PCR-amplified cco2 promoter ligated into pSEK103 using SpeI and XhoI.	this study
pLD2779	PCR-amplified ccoN4 promoter ligated into pSEK103 using SpeI and XhoI.	this study

elife-31250-v2.xml

10.7554/eLife.31250.023

Table 1.Plasmids used in this study

Plasmid	Description	Source or reference
YCplac111	sc LEU2 cloning vector	(Gietz and Sugino, 1988)
YEplac181	hc LEU2 cloning vector	(Gietz and Sugino, 1988)
YCplac22	sc TRP1 cloning vector	(Gietz and Sugino, 1988)
p3390/pDSO9	sc LEU2 TIF11 in YCplac111	(Choi et al., 2000)
p5633	sc LEU2 tif11-K3E in YCplac111	This study
p5635	sc LEU2 tif11-K4D in YCplac111	This study
p5638	sc LEU2 tif11-T6D in YCplac111	This study
p5637	sc LEU2 tif11-T6R in YCplac111	This study
p5640	sc LEU2 tif11-ΔG8 in YCplac111	This study
p5642	sc LEU2 tif11-R13P in YCplac111	This study
p5644	sc LEU2 tif11-G15D in YCplac111	This study
pDH469	sc LEU2 tif11-K7A in YCplac111	This study
pDH468	sc LEU2 tif11- K7D in YCplac111	This study
pDH481	sc LEU2 tif11-ΔG8ΔG9 in YCplac111	This study
pDH471	sc LEU2 tif11-K10A in YCplac111	This study
pDH470	sc LEU2 tif11-K10D in YCplac111	This study
pDH473	sc LEU2 tif11-R13A in YCplac111	This study
pDH472	sc LEU2 tif11-R13D in YCplac111	This study
pDH475	sc LEU2 tif11-R14A in YCplac111	This study
pDH474	sc LEU2 tif11-R14D in YCplac111	This study
pDH478	sc LEU2 tif11-K16A in YCplac111	This study
pDH476	sc LEU2 tif11-K16D in YCplac111	This study
p3400/pDSO23	hc LEU2 TIF11 in YEplac181	(Choi et al., 2000)
pPMB167	hc LEU2 tif11-K4D in YEplac181	This study
pPMB168	hc LEU2 tif11-ΔG8 in YEplac181	This study
pPMB169	hc LEU2 tif11-ΔG8ΔG9 in YEplac181	This study
pPMB170	hc LEU2 tif11- K10D in YEplac181	This study
p4281/YCpTIF5-G31R-W	sc TRP1 TIF5-G31R in YCplac22	(Valásek et al., 2004)
p4280/YCpSUI3-S264Y-W	sc TRP1 SUI3-S264Y in YCplac22	(Valásek et al., 2004)
p367	sc URA3 HIS4(ATG)-lacZ	(Donahue and Cigan, 1988)
p391	sc URA3 HIS4(TTG)-lacZ	(Donahue and Cigan, 1988)
p180	sc URA3 GCN4-lacZ	(Hinnebusch, 1985)
pPMB24	sc URA3 SUI1-lacZ	(Martin-Marcos et al., 2011)
pPMB25	sc URA3 SUI1-opt-lacZ	(Martin-Marcos et al., 2011)
pPMB28	sc URA3 SUI1_UUU-lacZ	(Martin-Marcos et al., 2011)
pC3502	sc URA3 ^-3AAA⁻¹ el.uORF1 GCN4-lacZ in YCp50	(Visweswaraiah et al., 2015)
pC4466	sc URA3 ^-3UAA⁻¹ el.uORF1 GCN4-lacZ in YCp50	(Visweswaraiah et al., 2015)
pC3503	sc URA3 ^-3UUU⁻¹ el.uORF1 GCN4-lacZ in YCp50	(Visweswaraiah et al., 2015)
pC3505	sc URA3 el.uORF1-less GCN4-lacZ in YCp50	(Visweswaraiah et al., 2015)
pTYB2-TIF11	TIF11 in pTYB2	(Acker et al., 2007)
p6013	tif11-R13P in pTYB2	This study
p6015	tif11-K16D in pTYB2	This study
pRaugFFuug	Dual luciferase reporter LUC_ren(aug)-LUC_firefly (uug) in URA3 vector	(Kolitz et al., 2009)
pRaugFFuug	Dual luciferase reporter LUC_ren(aug)-LUC_firefly (uug) in URA3 vector	(Kolitz et al., 2009)

elife-31981-v2.xml

10.7554/eLife.31981.016

Table 2.Plasmids used in this study

Plasmid	Description	Reference or source
pFL38	CEN-ARS (URA3)	(Bonneaud et al., 1991)
pFL36	CEN-ARS (LEU2)	(Bonneaud et al., 1991)
p416 GAL1	CEN-ARS GAL1p (URA3)	(Mumberg et al., 1994)
pJOD10	p416 GAL1p-GAP1-GFP (URA3)	(Nikko et al., 2003)
pCJ038	p416 GAL1p -GAP1(K9R,K16R)-GFP (URA3)	(Lauwers and André, 2006)
pMA065	p416 GAL1p -GAP1-126-GFP (URA3)	(Merhi et al., 2011)
pMA091	p416 GAL1p -GAP1-126-K9R,K16R-GFP (URA3)	(Merhi et al., 2011)
pCH500	CEN-ARS-GAP1 (URA3)	(Hein and André, 1997)
pHcGAP1	YEp-HcGAP1 (URA3)	(Wipf et al., 2002)
pES103	YEp-HA-NPR1 (LEU2)	This study
pAS103	YEp-HA-NPR1 (URA3)	(Schmidt et al., 1998)
pCJ315	CEN-ARS (LEU2-HIS3-LYS2)	Lab collection
pMYC008	YCp-AGP1p-LACZ (HIS3 TRP1 LEU2)	Lab collection
pES154	YCp-AGP1p-LACZ (HIS3 TRP1)	This study
pHl-U	YEp-ADH1p-pHluorin (URA3)	(Orij et al., 2009; Zimmermannova et al., 2015)
pHl-I	YCp-loxP-kanMX-loxP-GPD1p-pHluorin (URA3)	(Orij et al., 2009; Zimmermannova et al., 2015)
pCJ366	YEp (TRP1-LEU2-HIS3)	Lab collection
pPS15-P1	YCp-(Sc)PMA1 (LEU2)	(Supply et al., 1993)
pPMA4882ochre	YEp-PMA1p-(Np)PMA4^882ochre (LEU2)	(Luo et al., 1999)

elife-32057-v1.xml

10.7554/eLife.32057.033

Appendix 1—table 2.Plasmids used in this study

Plasmid	Relevant genotype/markers	Source
pGM074	pKASBAR-kan (Bottomley et al., 2014) carrying ezra-psmorange under the putative ezrA promoter (amp, kan)	G. McVicker
pSNAP-tag (T7)−2	E. coli expression plasmid carrying the snap gene under the control of the T7 promoter (amp)	New England Biolabs
pOB	pGEM3Zf(+) cloning vector containing the erythromycin resistance cassette (amp, ery)	(Horsburgh et al., 2002a)
pAISH	TetR derivative of pMUTIN4	(Aish, 2003)
pKASBAR-EzrA-eYFP	pKASBAR-kan containing ezrA-eyfp under the putative ezrA promoter (amp, kan)	This study
pKASBAR-EzrA-meYFP	pKASBAR-kan containing ezrA-meyfp under the putative ezrA promoter (amp, kan)	This study
pKASBAR-EzrA-GFP	pKASBAR-kan containing ezrA-gfp under the putative ezrA promoter (amp, kan)	This study
pKASBAR-EzrA-SNAP	pKASBAR-kan containing ezrA-snap under the putative ezrA promoter (amp, kan)	This study
pOB-ΔezrA	pOB containing the ezrA deletion cassette consisting of a 1.5 kb fragment of the upstream region of S. aureus ezrA, the tetracycline resistance cassette from pAISH and a 1.5 kb fragment of the downstream region of S. aureus ezrA (amp, ery, tet)	This study
pSS26b	pUC19 encoding snap (amp)	Covalys
pSS26bFtsZ-C	pSS26b containing ftsZ-snap (amp)	This study
pCQ11	E. coli-S. aureus shuttle vector containing lacI, Pspac and gfp (amp, ery)	(Hardt et al., 2017)
pCQ11-FtsZ-SNAP	pCQ11 derivative containing ftsZ-snap under Pspac (amp, ery)	This study
pCQ11-FtsZ-eYFP	pCQ11-FtsZ-SNAP with eyfp replacement of snap (amp, ery)	This study

elife-35588-v2.xml

10.7554/eLife.35588.026

Table 1.Plasmids used in this study.

Genes are human unless otherwise stated

Plasmid	Backbone	Insert	Ref
APX1-GFP1-10	APX1	super-folder GFP	This study
CDV-hyPBase	pigg	piggyBAC transposase	(Yusa et al., 2011)
NES-EGFP-P4M × 1	pEGFP-C1	X.leavis map2k1.L(32-44):EGFP:L. pneumophila SidM(546-647)	This study
FKBP-mCherry	pmCherry-N1	FKBP1A(isoform a, 3–108):mCherry	This study
SAC1^∆TMD-FKBP-mCherry	pmCherry-N1	SACM1L(1-521):FKBP1A(3-108):mCherry	This study
SAC1^C389S∆TMD-FKBP-mCherry	pmCherry-N1	SACM1L(C389S; 1–521):FKBP1A(3-108):mCherry	This study
iRFP-Sec61β	piRFP-C1	iRFP:SEC61B	This study
mKO-ManII	pmKO-N1	Kusabira Orange 2:Man2a(1-102)	Tamas Balla
mCherry-VAPB	pmCherry-C1	mCherry:VAPB	This study
mCherry-MAPPER	pmCherry-C1	mCherry:MAPPER	(Chang et al., 2013)
EGFP-MAPPER	pEGFP-C1	EGFP:MAPPER	(Chang et al., 2013)
GFP-ORP5	pEGFP-C1	EGFP:OSBPL5(isoform a)	(Sohn et al., 2016)
GFP-E-Syt2	pEGFP-C1	EGFP:ESYT2	(Giordano et al., 2013) Addgene plasmid #66831
EGFP-SAC1	pEGFP-C1	EGFP:SACM1L	(Sohn et al., 2016)
mEmerald-N16-Calreticulin	pmEmerald-N1	mEmerald:CALR	Michael Davidson (Addgene plasmid #54023)
GFP-Sec61β	pAcGFP-C1-Sec61β	Aequorea coerulescens GFP:SEC61B	(Voeltz et al., 2006) Addgene plasmid #15108
EGFP-E-Syt1	pEGFP-C1	EGFP-ESYT1	(Giordano et al., 2013) Addgene plasmid #66830
EGFP-STIM1	pEGFP-C1	STIM1(isoform 1 1–22):EGFP:STIM1(23–791)	(Várnai et al., 2007)
EGFP-Nir2	pEGFP-N1	EGFP:PITPNM1(isoform 2)	(Kim et al., 2015)
EGFP-P4M × 2	pEGFP-C1	EGFP:L. pneumophila SidM(546-647):SidM(546-647)	(Hammond et al., 2014)
Lyn₁₁-FRB-iRFP	piRFP-N1	LYN(1-11):MTOR(2021–2113):iRFP	(Hammond et al., 2014)
mCherry-FKBP	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG	(Hammond et al., 2014)
mCherry-FKBP-SAC1	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L	This study
mCherry-FKBP-SAC1^C389S	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L^C389S	This study
mCherry-SAC1-FKBP	pmCherry-C1	mCherry:SACM1L:FKBP1A(3-108)	This study
mCherry-SAC1^C389S-FKBP	pmCherry-C1	mCherry:SACM1L^C389S:FKBP(3-108)	This study
SAC1^∆452-587-FKBP-mCherry	pmCherry-N1	SACM1L(1-451):FKBP1A(3-108):mCherry	This study
mCherry-FKBP-SAC1^∆TMD	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L(1-521)	This study
mCherry-FKBP-SAC1-HLx2	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG::SACM1L(1-520):[EAAAR]₂:SACM1L(521-587)	This study
mCherry-FKBP-SAC1-HLx4	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG::SACM1L(1-520):[EAAAR]₄:SACM1L(521-587)	This study
mCherry-FKBP-SAC1-HLx6	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L(1-520):[EAAAR]₆:SACM1L(521-587)	This study
mCherry-FKBP-SAC1-HLx8	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L(1-520):[EAAAR]₈:SACM1L(521-587)	This study
mCherry-FKBP-SAC1-HLx10	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L(1-520):[EAAAR]₁₀:SACM1L(521-587)	This study
mCherry-FKBP-SAC1^C389S-HLx8	pmCherry-C1	mCherry:FKBP1A(3-108):[GGSA]₄GG:SACM1L^C389S(1-520):[EAAAR]₈:SACM1L(521-587)	This study
mCherry	pmCherry-C1	mCherry	(Hammond et al., 2014)
mCherry-SAC1	pmCherry-C1	mCherry:SAC1ML	(Sohn et al., 2016)
mCherry-SAC1-HLx8	pmCherry-C1	mCherryLSACM1L(1-520):[EAAAR]₈:SACM1L(521-587)	This study
mCherry-SAC1^C389S-HLx8	pmCherry-C1	mCherry:SACM1L^C389S(1-520):[EAAAR]₈:SACM1L(521-587)	This study
mTagBFP2-FKBP-CYB5A^tail	pmTagBFP2-C1	mTagBFP2:FKBP1A(3-108):[GGSA]₄GG:CYB5A(100-134)	This study

elife-43826-v1.xml

10.7554/eLife.43826.020

Table 1.Plasmids used in this study.

Name	Description	Vector-Promoter	Addgene
P1	HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)	pAAV-CMV	125224
P2	gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)	pAAV-CMV	125225
P3	mCherry-GSGS linker-Zdk1	pAAV-CMV	125226
P4	DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5	pAAV-CMV	125227
P5	AVPR2-NNES-eLOV-TEVcs-Flag-Gal4-V5	pAAV-CMV	104844
P6	β2AR-NNES-eLOV-TEVcs-Flag-Gal4-V5	pAAV-CMV	104841
P7	NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp	pAAV-CMV	125228
P8	UAS-Citrine	pAAV-UAS	104839
P9	βarrestin2-HA-GS linker-TEVp	pAAV-CMV	104845
P10	UAS-FLuc	pAAV-UAS	104840
P11	IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc	pAAV-CMV	125229
P12	gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4	pLX208-CMV	125230
P13	NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp	pLX208-CMV	125231
P14	UAS-Citrine	pFPGW-UAS	125232
P15	gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA	pLX208-CMV	125233
P16	gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5	pAAV-CMV	125234
P17	NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp	pAAV-CMV	125235
P18	β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5	pAAV-CMV	125236

elife-48215-v2.xml

10.7554/eLife.48215.013

Table 3.Plasmids used in this study.

Plasmid number	Genotype	Reference
pSMH145	pLIC-Tra His6-TEV-Ctf3; His6-TEV-Mcm16; His6-TEV-Mcm22	(Hinshaw et al., 2017)
pSMH1269	pFA6a-CTF3-6Gly-3FLAG-KanMX6 (BsiWI/SalI)	This study
pSMH1577	pFA6a-ctf3-SDD-6Gly-3FLAG-KanMX6 (BsiWI/SalI)	This study

elife-56582-v2.xml

Table 2.Plasmids used in this study.

Protein complex	Plasmid name	Names used in this paper	Proteins expressed^*	Vector	References
Mif2	Sc_Mf_7	Mif2	Mif2-linker-(27-392)MBP-6XHis**	pLIC	This study
	pGH52	Mif2	Mif2-linker-(27-392)MBP**	pLIC	This study
	Sc_Mf_5B	ΔN-Mif2	(41-549)Mif2-linker-(27-392)MBP	pLIC	This study
OA	pGH3	OA	Ame1-6XHis, Okp1	pST39	This study
	pGH4	OA	Ame1-FLAG, Okp1	pST39	This study
	pGH42	ΔN-OA	(21-324)Ame1-FLAG, Okp1	pST39	This study
	pGH15	ΔN-OA	(21-324)Ame1-6XHis, Okp1	pST39	This study
MIND	pGH63	2D-MIND	6XHis-linker-Nsl1, S240D, S250D-Dsn1, Mtw1, Nnf1	pST39	This study
	pGH62	2D-MIND	FLAG-Nsl1, S240D, S250D-Dsn1, Mtw1, Nnf1	pST39	This study
	pGH46	MIND	Nsl1, FLAG-Dsn1, Mtw1, Nnf1	pST39	This study
Ndc80c	pJT048	Part of Ndc80c	Spc24-Flag, Spc25	pRSFDuet	Kudalkar et al., 2015
	pEM033	Part of Ndc80c	Spc24-6XHis, Spc25	pRSFDuet	Scarborough et al., 2019
	Ndc80/Nuf2	Part of Ndc80c	Nuf2, Ndc80	pETDuet	Wei et al., 2005
Dam1c	pJT044	Dam1c	Dad1, Duo1, Spc34-FLAG, Dam1, Hsk3 and Dad4, Dad3, Dad2, Spc19, Ask1^‡	pST39	Umbreit et al., 2014
CI	pGH58	CI	FLAG-Chl4, Iml3	pLIC	This study
Histones	pScKl2	Cse4-NCP	K.lactis 6XHis-H2A, K. lactis 6XHis-H2B, Cse4, K. lactis 6XHis-H4	pLIC	Migl et al., 2020
	pScKl4	H3-NCP	H3, 6XHis-H2A, H2B. K.lactis 6XHis-H4	pLIC	Migl et al., 2020
	pScHT4	Cse4^(1-50)	6XHis-MBP-(1-50)Cse4	pLIC	This study