plasmids (3)

elife-36349-v2.xml

10.7554/eLife.36349.015

Table 2.Plasmids

Name	Description	Source
ops variants
pIA1087	P_BAD‒ops^WT‒luxCDABE	Burmann et al. (2012)
pZL6	P_BAD‒ops(G2C)‒luxCDABE	This work
pZL7	P_BAD‒ops(A7T)‒luxCDABE	This work
pZL12	P_BAD‒ops(T11G ‒luxCDABE	This work
pZL14	P_BAD‒ops(G5A)‒luxCDABE	This work
pZL21	P_BAD‒ops(G4C)‒luxCDABE	This work
pZL22	P_BAD‒ops(T6A)‒luxCDABE	This work
pZL23	P_BAD‒ops(G8C)‒luxCDABE	This work
pZL24	P_BAD‒ops(G12C)‒luxCDABE	This work
pZL25	P_BAD‒ops(G1C)‒luxCDABE	This work
pZL26	P_BAD‒ops(C3G)‒luxCDABE	This work
pZL27	P_BAD‒ops(C9G)‒luxCDABE	This work
pZL28	P_BAD‒ops(G10C)‒luxCDABE	This work
pIA1286	P_BAD‒ops(C3G + G8C)‒luxCDABE	This work
Gene expression vectors
pVS10	P_T7 promoter– E. coli rpoA–rpoB–rpoC^His6–rpoZ	Belogurov et al. (2007)
pVS12	E. coli rfaH in pTYB1	Vassylyeva et al. (2006)
pIA238	E. coli rfaH in pET28a	Artsimovitch and Landick (2002)

elife-37751-v2.xml

10.7554/eLife.37751.033

Table 3.Plasmids.

Plasmid name	Description
pcDNA5-FRT-TetO	Singh et al. (2012)
pcDNA5-FRT-TetO-fSNAPc	open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites
pcDNA5-FRT-TetO-U1-70K-fSNAPc	open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites
pcDNA5-FRT-TetO-U2B''-fSNAPc	open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites
pcDNA5-FRT-TetO-Snu114-fSNAPc	open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites
PIP85.A (=T7-5i3)	Moore and Sharp (1992), T7 transcription template for 5i3
T7-3e5	T7 transcription template for 3e5
T7-5iX	T7 transcription template for 5iX
T7-Xi3	T7 transcription template for Xi3
T7-3eX	T7 transcription template for 3eX
T7-Xe5	T7 transcription template for Xe5
T7-5i3e5	T7 transcription template for 5i3e5
T7-5i3eX	T7 transcription template for 5i3eX
T7-Xi3e5	T7 transcription template for Xi3e5
T7-Xi3eX	T7 transcription template for Xi3eX

elife-47791-v2.xml

10.7554/eLife.47791.021

Table 2.Plasmids.

Plasmid	Description	Type	Reference/source
pAM14	URA3 P_CYC1-4xSTRE-yNlucPEST	CEN/ARS	This work
pAM17	URA3 P_CYC1-3xHSE-yNlucPEST	CEN/ARS	This work
pAM23	URA3 P_THD3-yNlucPEST	CEN/ARS	This work
pCA502	HIS3 VC	CEN/ARS	(Andréasson et al., 2008)
pCA503	HIS3 SSE1	CEN/ARS	(Andréasson et al., 2008)
pCA901	HIS3 SSE1*-NLS	CEN/ARS	This work
pCA926	HIS3 SSE1-NLS	YIP	This work
pCA955	URA3 P_CYC1-HSE-yNlucPEST	CEN/ARS	(Masser et al., 2016)
pCA970	HIS3 SSE1-NES	CEN/ARS	(Kaimal et al., 2017)
pCA1026	P_T7-lacO-6xHis-SUMO-Ssa1-S/D-Hsf1-StrepTag II P_T7-Sis1 lacI	E. coli	This work
pJK001	HIS3 ysfGFP-SSE1	CEN/ARS	(Kaimal et al., 2017)
pJK010	HIS3 SSE1-NLS	CEN/ARS	(Kaimal et al., 2017)
pJK011	HIS3 ysfGFP-Sse1-NLS	CEN/ARS	This work
pJK070	URA3 SSA1_E423TAG-HA	2 μ	(Gowda et al., 2018)
ECYRS-BpA	TRP1 BPa system	2 μ	(Chen et al., 2007)