primers used in this study (6)

elife-30205-v1.xml

10.7554/eLife.30205.017

Table 2.Primers used in this study.

Primer number	Sequence	used to make plasmid number
LD717	ccaggcaaattctgttttatcagaccgcttctgcgttctgatCAGGACAAGCAGTGGGAAC	pLD1852
LD718	aggtgttgtaggccatcagcTGGCGGACCACCTTATAGTT
LD958	aactataaggtggtccgccaCGGTGGTTTCTTCCTCACC
LD959	ggaattgtgagcggataacaatttcacacaggaaacagctGGTCCAGCCTTTTTCCTTGT
LD725	ccaggcaaattctgttttatcagaccgcttctgcgttctgatCCCCTCAGAGAAGTCAGTCG	pLD1610
LD726	aggtgttgtaggccatcaggGGCGGACCACCTTGTAGTTA
LD727	taactacaaggtggtccgccCCTGATGGCCTACAACACCT
LD728	ggaattgtgagcggataacaatttcacacaggaaacagctCAGCGGGTTGTCATACTCCT
LD741	ccaggcaaattctgttttatcagaccgcttctgcgttctgatTCGAGGGCTTCGAGAAGAT	pLD1616
LD742	aggtgttgtaggccatcagcCAGGGTCATCAGGGTGAACT
LD743	agttcaccctgatgaccctgGCTGATGGCCTACAACACCT
LD744	ggaattgtgagcggataacaatttcacacaggaaacagctCGGGTGATGTCGACGTATTC
LD438	ggaattgtgagcggataacaatttcacacaggaaacagctCCGTTGATTTCCTTCTGCAT	pLD1264 (LD438 - LD441) pLD1853 (LD438 and LD441)
LD439	ctacaaggtggttcgccagtCGCTGACCTACTCCTTCGTC
LD440	gacgaaggagtaggtcagcgACTGGCGAACCACCTTGTAG
LD441	ccaggcaaattctgttttatcagaccgcttctgcgttctgatCATCGACCTGGAAGTGCTC
LD725	ccaggcaaattctgttttatcagaccgcttctgcgttctgatCCCCTCAGAGAAGTCAGTCG	pLD1929
LD1063	gttgcccaggtgttcctgtGGCGGACCACCTTGTAGTTA
LD949	ggaattgtgagcggataacaatttcacacaggaaacagctTGTAGTCGAGGGACTTCTTGC
LD1064	taactacaaggtggtccgccACAGGAACACCTGGGCAAC
LD2168	ccaggcaaattctgttttatcagaccgcttctgcgttctgatATGTAGGGATCGAGCGACAG	pLD2791
LD2169	acacgatatccagcccctctTGGACATCGCGCCGTTCCTC
LD2170	gaggaacggcgcgatgtccaAGAGGGGCTGGATATCGTGT
LD2171	ggaattgtgagcggataacaatttcacacaggaaacagctAAGAGGTCATAATCGGCGGT
LD2120	gattcgacatcactagtACGCCCAGCTCCAACAAA	pLD2777
LD2121	gattcgatgccctcgaGCTAGGGGTTCCACGGTTAAT	pLD2777
LD2122	gattcgactgcactagtCATCGACTTGCCGCCCAG	pLD2778
LD2123	g attcg atg ccctcgaGCTATGGGCTTCCATC CAC	pLD2778
LD2124	gattcgactgcactagtGGCTACTTCCTCTGGCTGG	pLD2779
LD2125	gattcgactgcctcgagCTGTACAGTCCCGAAAGAAATGAAC	pLD2779
LD1118	ccaggcaaattctgttttatcagaccgcttctgcgttctgatTCTTCAGGTTCTCGCGGTAG	pLD1966
LD1119	aagtgccagtaccaactggcGCAGATCCAGAAGATGGTCA
LD1120	tgaccatcttctggatctgcGCCAGTTGGTACTGGCACTT
LD1121	ggaattgtgagcggataacaatttcacacaggaaacagctATCGCGAGACTCATGGTTTT
LD1134	ccaggcaaattctgttttatcagaccgcttctgcgttctgatCGCTGCTTGTCGATCTGTT	pLD1967
LD1135	gcgacatgaccctgttcaacCTGACCGGCTACTGGACC
LD1136	ggtccagtagccggtcagGTTGAACAGGGTCATGTCGC
LD1137	ggaattgtgagcggataacaatttcacacaggaaacagctCCTCGGCGACCATGAATAC
LD1126	ccaggcaaattctgttttatcagaccgcttctgcgttctgatTTCAGGTTCTTCGGGTTCTC	pLD2044
LD1187	aacagcgcgccgaccagcatCTCTTCGTTCGTTTTCAGCC
LD1188	ggctgaaaacgaacgaagagATGCTGGTCGGCGCGCTGTT
LD1189	ggaattgtgagcggataacaatttcacacaggaaacagctGCGTTGATGAAGCGGATAAC

elife-37419-v2.xml

10.7554/eLife.37419.027

Table 1.Primers used in this study.

F = forward primer, R = reverse primer.

Gene symbol	Sequence
Srsf3	F	TGAATTAGAACGGGCTTTTGG
Srsf3	R	TTCACCATTCGACAGTTCCAC
Hprt	F	TGTTGTTGGATATGC
Hprt	R	TGCGCTCATCTTAGG
Nanog	F	ACCAAAGGATGAAGTGCAAGC
Nanog	R	TGGATGCTGGGATACTCCACT
Nanog TR	F	GCAGTTTTTCATCCCGAGAAC
Nanog TR	R	GAAGAGGCAGGTCTTCAGAGG
Nanog FL	F	TGACATGAGTGTGGGTCTTCC
Nanog FL	R	GAAGAGGCAGGTCTTCAGAGG
Pou5f1	F	GAGGAAGCCGACAACAATGAG
Pou5f1	R	5'ATCTGCTGTAGGGAGGGCTTC
Sox2	F	GTAAGATGGCCCAGGAGAACC
Sox2	R	ATAATCCGGGTGCTCCTTCAT
Klf4	F	GAAAAGAACAGCCACCCACAC
Klf4	R	CCTGTCACACTTCTGGCACTG
Zfp42	F	AGATTAGCCCCGAGACTGAGG
Zfp42	R	AAGGGAACTCGCTTCCAGAAC
Myc	F	CTGTACCTCGTCCGATTCCAC
Myc	R	GGTTTGCCTCTTCTCCACAGA
Nxf1	F	TTCTGCCTGTCTGTTGTCTCC
Nxf1	R	CAGAACAGAAAAGGGGAGGTG
Mbnl2	F	AAAGCACTGAAGCGACCTCTC
Mbnl2	R	AGAGCCTGCTGGTAGTGCAAG
Ewsr1	F	GCTTCAATAAGCCTGGTGGAC
Ewsr1	R	TGCCAGATCATCCAGAGTCAC
Fil1l1	F	TCCAATAACTGTACCACCTCCA
Fil1l1	R	CCATAGGGAACGCTCGTG
Ezh2 Ex2-3	F	AATCTGAGAAGGGACCGGTTT
Ezh2 Ex2-3	R	ATGTGCACAGGCTGTATCCTC
Ezh2 Ex19-20	F	GGGCTATCCAGACTGGTGAAG
Ezh2 Ex19-20	R	CCTGAAGCTAAGGCAGCTGTT
Epop	F	CCGGCTGATGCTCTTTCTACT
Epop	R	CCGCTAAACTGACCCTCATTC
Eed	F	GGCAAACTGTATGTTTGGGATT
Eed	R	TCGCAGACAGCTATGAGGATG
Wee1	F	GAGCTGGTGAAGCATTCAGTG
Wee1	R	CATCCGATCTGTGAAGAGTGC
Dnmt3a Ex22-23	F	GGGGACCCCTACTACATCAGC
Dnmt3a Ex22-23	R	AGAGGCCTGGTTCTCTTCCAC
Dnmt3a AltEx1	F	CCAGACGGGCAGCTATTTACA
Dnmt3a AltEx1	R	AGAGGCCTGGTTCTCTTCCAC
Nanog-WT and Nanog-ΔSRSF3	F	CAAGCCTCAGACAGTGGTTCA
Nanog-WT and Nanog-ΔSRSF3	R	ATGTCAGTGTGATGGCGAGG

elife-42834-v2.xml

10.7554/eLife.42834.028

Table 1.Primers used in this study.

Purpose	Primer name	Sequence (5'−3')
Genotyping
rec8 T_DNA	SAIL_LB3	TAGCATCTGAATTTCATAACCAATCTCGATACAC
	SAIL_807_B08-RP	GGGGGAAAAGAGAAAGGTTC
rec8 WT allele	SAIL_807_B08-LP	CTCATATTCACGGTGCTCCC
	SAIL_807_B08-RP	GGGGGAAAAGAGAAAGGTTC
rec8 WT allelle in REC8:GFP	SAIL_807_B08-RP	GGGGGAAAAGAGAAAGGTTC
	TL-gREC8-R	GAACGGAGAAGGGTAAGGCTCTTGAGTC
tam T_DNA	SAIL_LB3	TAGCATCTGAATTTCATAACCAATCTCGATACAC
	TAM_L	CAGAAATCCTCCACTTGCG
tam WT allele	TAM_U	GACTTGATGGATCCACAGC
	TAM_L	CAGAAATCCTCCACTTGCG
Cloning of PRO_REC8:REC8:GFP
REC8 genome	AT5G05490-F	CACCCCAGCCAAGACATTGTGATCTTCAAC
REC8 genome	AT5G05490-R	TGTGTGATTCAGGGGTAAGAAATATGCG
SmaI	REC8 CterSmaI-F	GGGTAAGGTTTGATTTCTAAATTA
SmaI	REC8 CterSmaI-R	GGGCATGTTGGGTCCTCTTGCAAT
locus of insertion of gREC8-GFP	gREC8-GFP_LP	GAATATTACCTTGCCATAGGCTTG
locus of insertion of gREC8-GFP	attB1r REC8ter-R	GGGGACTGCTTTTTTGTACAAACTTGTGTGTGATTCAGGGTAAGAAA
	attB4 REC8_2ndI-F	GGGGACAACTTTGTATAGAAAAGTTAATCAACTCAATTCCCTGTG
	attB1r REC8_2ndI-R	GGGGACTGCTTTTTTGTACAAACTTGGCAAAGAGATAAAACCACGC
	REC8 2nd intron-F	GCCGCCCCCTTCACCGTAATCAACTCAATTCCCTG
	REC8 2nd intron-R	TTCGAATTCCGTTACCTGCAAAGAGATAAAACCAC
	Vector for REC8 2I-F	CTCTTTGCAGGTAACGGAATTCGAAATTTA
	Vector for REC8 2I-R	AGTTGATTACGGTGAAGGGGGCGGCCGCGG
Cloning of PRO_REC8:REC8:GFP/TagRFP:PRO_RPSA5:TUA5
	attB4 TUA5-F	GGGGACAACTTTGTATAGAAAAGTTTTGATTCGCTATTTGCAGTGCAC
	attB1r TUA5-R	GGGGACTGCTTTTTTGTACAAACTTGTGTGTGATTCAGGGGTAAGAAA

elife-52482-v2.xml

Table 3.Primers used in this study.

Primer name	Sequence (5’ to 3’)
AmgK_BamHI_F	GATAGGATCCTGACCCGCTTGACGGCTA
MurU_HindIII_R	GTATAAGCTTTCAGGCGCGCTCGC
RdxA_F1P1	CAATTGCGTTATCCCAGC
RdxA_dnstm_RP2	AAGGTCGCTTGCTCAATC
O#9 ProMreB (KpnI_5’)	TATTGGTACCCGCTTGATGTATTCATCAAAG
O#10 ProMreB_R	GATTAATTTGCTAAAAATCATAAAATAAACTCCTTGTTTTG
O#11 ProMreB_F	CAAAACAAGGAGTTTATTTTATGATTTTTAGCAAATTAATC
O#12 ProMreB (XhoI_3’)	TATTCTCGAGTTATTCACTAAAACCCACAC
O#36 pMcGee-Insert-F	CTGCCTCCTCATCCTCTTCATCCTC
O#45 MreBC-seq-F2	GCACCTATTTTGGGGTTTGAAACC
O#47 MreB-seq-F2	CATTGAGCGCTGGTTTTAAGGCGGTC
O#28 MreBseq-F3	CGATCGTGTTAGTCAAAGGGCAGGGC
O#37 pMcGee-Insert-R	GGTGTACAAACATTTAAAGGTAGAG
O#68 McGee-1F	CATTTCCCCGAAAAGTGCCACGAGCTCGAAGGAGTATTGATGAAAAAGG
O#69 McGee-1R	CTAGAGCGGCCCCACCGCGGCCATCATTAACATCATTATCG
O#70 MCS-kan-F	CTCGAGGGGGGGCCCGGTACCCACAGAATTACTCTATGAAGC
O#71 MCS-kan-R	CCATTCTAGGCACTTATCCCCTAAAACAATTCATCCAGTAA
O#72 McGee-2F	TTACTGGATGAATTGTTTTAGGGGATAAGTGCCTAGAATGG
O#73 McGee-2R	CGGATATTATCGTGAGATCGCTGCAGACTGGGGGGAAACTCATGGG
O#74 McGee-R6K-F	CCCATGAGTTTCCCCCCAGTCTGCAGCGATCTCACGATAATATCCG
O#75 McGee-R6K-R	GTAACTGTCAGACCAAGTTTACTGCGGCCGCGCAAGATCCGGCCACGATGCG
O#76 R6K-amp-F	CGCATCGTGGCCGGATCTTGCGCGGCCGCAGTAAACTTGGTCTGACAGTTAC
O#77 R6K-amp-R	CCTTTTTCATCAATACTCCTTCGAGCTCGTGGCACTTTTCGGGGAAATG
O#78 MCS fragment	CCGCGGTGGGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCGGAATTCGCTTATCG
O#79 McGee-MCS-F	CGATAATGATGTTAATGATGGCCGCGGTGGGGCCGCTCTAG
O#80 McGee-MCS-R	GCTTCATAGAGTAATTCTGTGGGTACCGGGCCCCCCCTCGAG
Csd1F	GAGTCGTTACATTAATGTGCATATCT
G1480_DnStrmP2	AAGGGTGCAATAACGCGCTAA
MreB_start_F	ATGATTTTTAGCAAATTAATCGG
MreB_cat_up_R	CACTTTTCAATCTATATCCGTGCCTCCGCCAATATC
C1	GATATAGATTGAAAAGTGGAT
C2	TTATCAGTGCGACAAACTGGG
Cat_mreB_dn_F	AGTTTGTCGCACTGATAAACTGAAATTGGCG
MreB_end_R	TTATTCACTAAAACCCACACGGCTGA
FabZ_up_F	GCTATCCCATGCTATTGATAGAC
Cat_mid_R	GTCGATTGATGATCGTTGTAACTCC
MreB_mid_dn_F	GATCAAAGCATCGTGGAATACATCC
Supp2_junc1_R_mid	AATTTGCTAAAAATCACTAA
MreB_up	AATACCAGCAACTTTTCAAAA
Supp1_Junction1_R	ATTTGCTAAAAACACACGGC
Catout	CCTCCGTAAATTCCGATTTGT
McGee_187	GCGAGTATTACCACAAGTTTTC
CcmA SDM mi R	AGACTAGATTGGATCATTCCCTATTTATTTTCAATTTTCT
CcmA SDM mi F	ATAAAGAAAGGAGCATCAGATGGCAATCTTTGATAACAAT
CcmA SDM up R	ATTGTTATCAAAGATTGCCATCTGATGCTCCTTTCTTTAT
CcmA SDM dn F	AGAAAATTGAAAATAAATAGGGAATGATCCAATCTAGTCT
CcmA SDM dn R	GCTCATTTGAGTGGTGGGAT
SDM 155A F	ATTCTAAAAGCACGGTGGTGgcCGGACAAACCGGCTCGGTAG
SDM 155A R	CTACCGAGCCGGTTTGTCCGgcCACCACCGTGCTTTTAGAAT
SDM L110S F	TGGTGGAAAGGAAGGGGATTtcGATTGGGGAAACTCGCCCTA
SDM L110S R	TAGGGCGAGTTTCCCCAATCgaAATCCCCTTCCTTTCCACCA

elife-52741-v1.xml

Table 2.Primers used in this study.

Capital letters in SLIC and Gibson primers indicate template binding regions.

Plasmid creation	Target amplicon (Source DNA)	Primer name	Primer sequence (5’→ 3’)
pEM01	CMV promoter (pAB1T7)	CMV_F	ccctcactaaagggaacaaaagctggagctgagctcGACATTGATTATTGACTAGTTATTAATAG
pEM01	CMV promoter (pAB1T7)	CMV_R	ctttttacccatgttaattaaAGCTCTGCTTATATAGACCTCC
pEM01	hph (pRS306H)	Hyg_F	tatataagcagagctttaattaacATGGGTAAAAAGCCTGAACTC
pEM01	hph (pRS306H)	Hyg_R	tagaagtggcgcgccaTTATTCCTTTGCCCTCGGAC
pEM01	ADH1 terminator (pNB780)	ADH1t_F	agggcaaaggaataatggcgcgccACTTCTAAATAAGCGAATTTCTTATG
pEM01	ADH1 terminator (pNB780)	ADH1t_R	tgacccggcggggacgaggcaagctaaacaATATTACCCTGTTATCCCTAGC
pEM03	Hsp70 promoter (Spun gDNA)	HSP70_F	actaaagggaacaaaagctggagctgagctcTTTTAAAATCTTGTCTTTGTGC
pEM03	Hsp70 promoter (Spun gDNA)	HSP70_R	ggtgagttcaggctttttacccatgttaattaaATTGTGCTGATCTTTGGTCC
pEM09	hph (pFA6-GFP(S65T)-hph)	HygR_F	aagatcagcacaatttaattaATGGGTAAAAAGCCTGAACTCAC
pEM09	hph (pFA6-GFP(S65T)-hph)	HygR_R	tcctcctcctcctccTTCCTTTGCCCTCGGACG
pEM09	GFP (pFA6-GFP(S65T)-hph)	GFP_F	gcaaaggaaggaggaggaggaggaggaAGTAAAGGAGAAGAACTTTTCAC
pEM09	GFP (pFA6-GFP(S65T)-hph)	GFP_R	cgcttatttagaagtggcgcgccTATTTGTATAGTTCATCCATGC
	Sequencing	pRS-up	aacataggagccggaagcataaagtg
	Sequencing	ADHt-dn	ctgccggtagaggtgtggtcaataag
pGI3EM9	Hsp70pr-hph-GFP (pEM09)	GI3EM9IIF	cgttgtaaaacgacggccagtgccaagcttttttaaaatcttgtctttgtgcac
pGI3EM9	Hsp70pr-hph-GFP (pEM09)	GI3EM9IIR	aggaaacagctatgacatgattacgaattcccggtagaggtgtggtcaataag
pGI3EM11	H2B promoter (Spun gDNA)	prH2B_F	cgttgtaaaacgacggccagtgccaagCTTTTATGCTCCAAGCGGAG
pGI3EM11	H2B promoter (Spun gDNA)	prH2B_R	gagttcaggctttttacccattaattaaTTTGTGTGTGTGATGGATGAG
pGI3EM11	HygR_up	GI3EM11up	ggatcctcctcctccTTCCTTTGCCCTCGGACG
pGI3EM18	tdTomato (pKT356)	HygtdTom_F	cagcactcgtccgagggcaaaggaaggaggaggaggatccATGGTGAGCAAGGGCGAG
pGI3EM18	tdTomato (pKT356)	AdhtdTom_R	tcgcttatttagaagtggcgcgcctTTACTTGTACAGCTCGTCCATG
pGI3EM20C	H2A/H2B promoter (Spun gDNA)	H2B2D_F	gctttttacccatttaattaatgctgtgtaaggtgtgcg
pGI3EM20B/C	H2A/H2B promoter (Spun gDNA)	H2B2D_R	gggtgccatgtcgacttgtgtgtgtgatggatgag
pGI3EM20B/C	H2B CDS (Spun cDNA)	H2Bgen_F	atcacacacacaagtcgacatggcacccaaggaagctc
pGI3EM20B/C	H2B CDS (Spun cDNA)	H2Bgen_R	gacctcctcgcccttgctcaccatggatccggaggaggatttagcagactggtacttcgtcac
pGI3EM20B/C	HygR (pEM03)	AdhHygF	cgttgtaaaacgacggccagtgccaagcttccggtagaggtgtggtcaataag
pGI3EM20B/C	HygR (pEM03)	AdhHygR	ccttacacagcattaattaaatgggtaaaaagcctgaactc
pGI3EM20B/C	invPCR T-DNA (LB)	4_LB_R	tgtggaattgtgagcggata
pGI3EM20B/C	invPCR/sequencing T-DNA (LB)	ai077_F	agaggcggtttgcgtattgg
pGI3EM22C	LifeAct (Synthesized DNA)	TwistLifeact_F	ctataaaaggcgggcgtgt
pGI3EM22C	LifeAct (Synthesized DNA)	TwistLifeact_R	gcgcatgaactctttgatga
pGI3EM29	mCitrine (mCitrine-PCNA-19-SV40NLS-4)	EM18Citrine_F	ccgagggcaaaggaaggaggaggaggatccATGGTGAGCAAGGGCGAG
pGI3EM29	mCitrine (mCitrine-PCNA-19-SV40NLS-4)	EM18Citrine_R	aagaaattcgcttatttagaagtggcgcgcCTTGTACAGCTCGTCCATGC
pGI3EM30	mClover3 (pKK-mClover3-TEV)	EM18Clover3_F	gagggcaaaggaaggaggaggaggatccATGGTGAGCAAGGGCGAG
pGI3EM30	mClover3 (pKK-mClover3-TEV)	EM18Clover3_R	gaaattcgcttatttagaagtggcgcgcCTTGTACAGCTCGTCCATGC
pGI3EM31	mCerulean3 (mCerulean3-N1)	EM18Cerulean3_F	ccgagggcaaaggaaggaggaggaggatccATGGTGAGCAAGGGCGAG
pGI3EM31	mCerulean3 (mCerulean3-N1)	EM18Cerulean3_R	aagaaattcgcttatttagaagtggcgcgccTTACTTGTACAGCTCGTCCATG
	mCitrine/Clover/Cerulean Sequencing	mClover_Down	gtccaagctgagcaaagacc
	PCR screen (Set C)/H2Bpr/Sequencing	H2BprF1	tttatgctccaagcggagac
	PCR screen (Set C)/Sequencing	TomR1	cttgtacagctcgtccatgc
	PCR screen (Set D)/H2Bpr/Sequencing	H2BprF2	cgttaaatgacctgctcgaa
	PCR screen (Set D)/H2Bpr/Sequencing	TomR2	ccatgccgtacaggaacag
	PCR screen (Set A)/Southern Blot/Sequencing	HygF1	gatgtaggagggcgtggata
	PCR screen (Set A)/Southern Blot/Sequencing	HygR1	atttgtgtacgcccgacagt
	PCR screen (Set B)/Sequencing	HygF2	gtcctgcgggtaaatagctg
	PCR screen (Set B)/Sequencing	HygR2	cgtctgctgctccatacaag

elife-56844-v2.xml

Table 1.Primers used in this study.

Purpose	Amplifies	Primers
PDR1 deletion	kanMX resistance cassette	5’-CAGCCAAGAATATACAGAAAAGAATCCAAGAAACTGGAAGCGTACGCTGCAGGTCGAC-3’ 5’-GGAAGTTTTTGAGAACTTTTATCTATACAAACGTATACGTATCGATGAATTCGAGCTCG-3’
PDR3 deletion	Nourseothricin resistance cassette	5’-ATCAGCAGTTTTATTAATTTTTTCTTATTGCGTGACCGCACGTACGCTGCAGGTCGAC-3’ 5’-TACTATGGTTATGCTCTGCTTCCCTATTTTCTTTGCGTTTATCGATGAATTCGAGCTCG-3’
GGA1 deletion	Hygromycin resistance cassette	5’-AGTCACTACTTCAAGTATAACCCAGACAAGAGTCTTTTAAATAGCTTGCCTTGTCCCCGC-3’ 5’-ATGGCATCTACTTTTTTTTCAACTTCTCTACCGAATTTGACGTTTTCGACACTGGATGGC-3’
VPS10 deletion	VPS10 5’ upstream	5’-CCCAAACTAAAAAGTATCCGCCTGT-3’ 5’-GACAAGGCAAGCTAACGTGTGATGACTACTGGACACT-3’
	VPS10 3’ downstream	5’-GCCATCCAGTGTCGAAGAGATTACTTTACATAGAGTAGATAATTCCATATACTTTTCATA −3’ 5’-AATGAAGTACTATAAATATTAAAGTACGTTAGTAGTTTATTTCTCTTCGG-3’
	Hygromycin resistance cassette	5’-TCATCACACGTTAGCTTGCCTTGTCCCCGC-3’ 5’-TGTAAAGTAATCTCTTCGACACTGGATGGCGG-3’
APM3 deletion	APM3 5’ upstream	5’-AGGGGTAGAAGTCGCTGATTGAT-3’ 5’-GGGCCTCCATGTCCTATTTTGGTTGGGTTGGTAAGGTTTACAG-3’
	APM3 3’ downstream	5’-GCTGGTCGCTATACTGTTATATGTGTACTTGAAATTCCATGCGAAACTAAA-3’ 5’-TGCGGAAGTCTTCCCTAAGACG-3’
	Hygromycin resistance cassette	5’-CAACCAAAATAGGACATGGAGGCCCAGAATACCC-3’ 5’-TCAAGTACACATATAACAGTATAGCGACCAGCATTCACA-3’
APL4 deletion	APL4 5’ upstream	5’-ATGTATATAATTCCGGAAGTGTGGTCCT-3’ 5’-GACAAGGCAAGCTTATGGTGTTCAGGTCTTTCTCGTTGCT-3’
	APL4 3’ downstream	5’-CCATCCAGTGTCGAAAAATGCCTTTAAAATTACAGAACATAACATGATTAATGAC-3’ 5’-GAATTCTGGTCCAAGGCAATTCTATATTTGAT-3’
	Hygromycin resistance cassette	5’-CCTGAACACCATAAGCTTGCCTTGTCCCCG-3’ 5’-TTTTAAAGGCATTTTTCGACACTGGATGGCGG-3’
GGA2 deletion	GGA2 5’ upstream	5’-GATTTCTACAGTCTTTCTGATGGGTTCTTGG-3’ 5’-ACGATATTCTTAGACATGATGCAGTATCACGATTAGCAAT-3’
	GGA2 3’ downstream	5’-AATCTTGGCTTAATCCTCTGGCGTTTCTTATCAATCCTTTCT-3’ 5’-TCTTCCTTTGAAGAAAATTCGTCCTCATCT-3’
	K. lactis LEU2	5’-AATCGTGATACTGCATCATGTCTAAGAATATCGTTGTCCTACCGG-3’ 5’-GAAACGCCAGAGGATTAAGCCAAGATTTCCTTGACAGCC-3’
Integration at TRP1	TRP1 locus	5’-GTGTACTTTGCAGTTATGACG-3’ 5’-AGTCAACCCCCTGCGATGTATATTTTCCTG-3’