qpcr primers used in this study (3)


elife-35388-v1.xml

10.7554/eLife.35388.014qPCR primers used in this study.
qPCR primers descriptionSequence
uncleaved Sp BiP1 mRNA forward primerGAATCGTGACTCTATAGCCATTAACA
uncleaved Sp BiP1 mRNA reverse primerCAATTATTGTCAGTTCCACAAAGC
total Sp BiP1 mRNA forward primerTGGTAAGGTTGATCCCGAAG
total Sp BiP1 mRNA reverse primerCATCGAGTTTTTGACGCTGA
Sp GAS2 mRNA forward primerGTTGTCAACAATGCCTCGAA
Sp GAS2 mRNA reverse primerCGGTCTCAGAGTTGGTGTCA
Sp NDA2 mRNA forward primerTCCATGAATCCAACAGCGTA
Sp NDA2 mRNA reverse primerCTAGTAACGGCAGCCTGGAC

elife-35407-v3.xml

10.7554/eLife.35407.016qPCR primers used in this study
qPCR primerSequence
RPS6 rightTGA TGT CCG CCA GTA TGT TG
RPS6 leftTCT TGG TAC GCT GCT TCT TC
TFAP2 rightATT GAC CTA CAG TGC CCA GC
TFAP2 leftATG CTT TGG AAA TTG ACG GA
PAX6 rightCAC ATG AAC AGT CAG CCA ATG
PAX6 leftGGC CAG TAT TGA GAC ATA TCA GG
EDNRA rightCAT GAC TTG TGA GAT GTT GAA CAG
EDNRA leftCTG TTT TTG CCA CTT CTC GAC
FOXD3 rightTTG ACG AAG CAG TCG TTG AG
FOXD3 leftTCT GCG AGT TCA TCA GCA AC
SIX3 rightCGA GGG GAG TGG ACA CTT
SIX3 leftATG CCG CTC GGT CCA AT
SNAI2 rightTGA CCT GTC TGC AAA TGC TC
SNAI2 leftCAG ACC CTG GTT GCT TCA A
SOX10 rightCTT TCT TGT GCT GCA TAC GG
SOX10 leftAGC TCA GCA AGA CGC TGG
FOXG1 rightTGA ATG AAA TGG CAA AGC AG
FOXG1 leftTGC AAT GTG GGG AGA ATA CA

elife-40526-v1.xml

10.7554/eLife.40526.023qPCR primers used in this study.
ATP5OACTCGGGTTTGACCTACAGCAAAATGAACGGACAGAACCG
BRATGCTTCCCTGAGACCCAGTTGATCACTTCTTTCCTTTGCATCAAG
CERACAGTGCCCTTCAGCCAGACTACAACTACTTTTTCACAGCCTTCGT
GATA3TTCCTCCTCCAGAGTGTGGTAAAATGAACGGACAGAACCG
HAND1GTGCGTCCTTTAATCCTCTTCGTGAGAGCAAGCGGAAAAG
ID2GCAGCACCTCATCGACTACAAATTCAGAAGCCTGCAAGGA
ID4CCCTCCCTCTCTAGTGCTCCGTGAACAAGCAGGGCGAC
LEFTY1ACCTCAGGGACTATGGAGCTCAGGAGAAATGGCCAATTGAAGGCCAGG
LHX1TCCCCAATGGTCCCTTCTCCGTAGTACTCGCTCTGGTAATCTCC
MIXL1CCGAGTCCAGGATCCAGGTACTCTGACGCCGAGACTTGG
NANOGCCGGTCAAGAAACAGAAGACCAGACCATTGCTATTCTTCGGCCAGTTG
NODALATGCCAGATCCTCTTGTTGGAGACATCATCCGCAGCCTAC
NOGCATGAAGCCTGGGTCGTAGTTCGAACACCCAGACCCTATC
OCT4GGGCTCTCCCATGCATTCAAACCACCTTCCCTCCAACCAGTTGC
SOX2CCATGCAGGTTGACACCGTTGTCGGCAGACTGATTCAAATAATACAG
TBR2/EOMESCACATTGTAGTGGGCAGTGGCGCCACCAAACTGAGATGAT
WNT3CTCGCTGGCTACCCAATTTGAGCCCAGAGATGTGTACTGC