yeast strains used in this study (9)

elife-30015-v2.xml

10.7554/eLife.30015.022

Table 1.Yeast strains used in this study.

Strain #	Strain name	Genotype	Reference
FA2	∆fzo1	FZO1::kanMX4 in BY4741	Brachmann et al., 1998
FA230	cdc48-1	cdc48-1::KanMX4 in BY4741	Li et al. (2011)
FA231	cdc48-2	cdc48-2::KanMX4 in BY4741	Li et al. (2011)
FA232	cdc48-3	cdc48-3::KanMX4 in BY4741	Li et al. (2011)
FA260	∆ubp2	UBP2::kanMX4 in BY4741	Brachmann et al., 1998
FA269	∆ubp12	UBP12::kanMX4 in BY4741	Brachmann et al., 1998
FA362	∆fzo1 ∆ubp2	FZO1::kanMX4; UBP2::kanMX4; obtained by crossing	Anton et al. (2013)
FA382	∆ubp2 ∆ubp12	UBP12::kanMX4; UBP2::kanMX4; obtained by crossing	Anton et al. (2013)
FA390	∆ubp12 ∆mdm30	UBP12::kanMX4; MDM30::kanMX4; obtained by crossing	this study
FA407	HA-Fzo1^int in wt	HA-Fzo1 genomically integrated with NatNT2 into RS140	Anton et al. (2013)
FA415	HA-Fzo1^int in ∆ubp2	HA-Fzo1 genomically integrated with NatNT2 into FA260	Anton et al. (2013)
FA427	HA-Fzo1^int in ∆ubp2 ∆mdm30	HA-Fzo1 genomically integrated with NatNT2 into ∆ubp2 ∆mdm30	Anton et al. (2013)
FA432	∆fzo1 ∆ubp12	FZO1::kanMX4; UBP12::kanMX4; obtained by crossing	this study
FA451	HA-Fzo1-K464R^int in wt	HA-Fzo1^K464R genomically integrated with NatNT2 into RS140	this study
RS140	wt	BY4741; S288C isogenic yeast strain; MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0	Brachmann et al., 1998
RS430	cdc48-2 ∆fzo1	FZO1::natNT2 in FA231	this study
RS466	cdc48-2 ∆ubp12	FZO1::hphNT1 in FA231	this study
RS499	cdc48-2 ∆ubp2 ∆ubp12	UBP12::natNT2; UBP2::hphNT1 in FA231	this study
RS518	∆doa1	DOA1::kanMX4 in BY4741	Brachmann et al., 1998
RS527	∆pdr5 ∆snq2	MATa, ADE2 his3-D200 leu2-3,112 lys2-801, trp1D63 ura3-52 PDR5::hphNT1 SNQ2::kanMX4	J. Dohmen (YGA58)
RS546	Ubp12-Flag^int in cdc48-2	Ubp12-Flag genomically integrated with NatNT2 into FA231	this study
RS547	Ubp12-Flag^int in wt	Ubp12-Flag genomically integrated with NatNT2 into BY4741	this study
RS554	∆pdr5 ∆snq2	PDR5::NatNT2; SNQ2::hphNT1 in RS140	this study
RS556	∆fzo1 ∆dnm1 ∆ubp12	UBP12::NatNT2 in TS1029	this study
RS559	∆pdr5 ∆snq2 cdc48-2	PDR5::NatNT2; SNQ2::hphNT1 in FA231	this study
TS686	cdc48-2 ∆ubp2	UBP2::hphNT1 in FA231	this study
TS1029	∆fzo1 ∆dnm1	FZO1::kanMX4; DNM1::kanMX4; Mat α, BY background, obtained by crossing	B. Westermann (#94)
TS1124	wt (DF5)	MATα, trp1-1(am), ura3-52, his3∆200, leu2-3, lys2-801	Hoppe et al. (2000)
TS1125	ufd1-2	ufd1-2^ts in TS1124	Braun et al. (2002)
TS1126	npl4-1	npl4-1^ts in TS1124	DeHoratius and Silver (1996)
TS1134	Ubp2-9Myc^int in wt	Ubp2-9Myc genomically integrated with NatNT2 into RS140	this study
TS1144	Ubp2-3HA^int in wt	Ubp2-3HA genomically integrated with hphNT1 in RS140	this study
TS1147	Ubp2-3HA^int in ∆ubp12	Ubp2-3HA genomically integrated with hphNT1 in FA269	this study
TS1153	pGAL-Ubp12-Flag^int in wt	pGAL-Ubp12-Flag genomically integrated with kanMX4 into RS544	this study

elife-30560-v2.xml

10.7554/eLife.30560.009

Table 2.Yeast strains used in this study.

Strain name	Relevant genotype	Source
E17	MATa ADE2 cdc21::kanMX leu2::LEU2-GAL-hENT1 LYS2 RAD5 trp1::TRP1-GAL-dNK ura3-1	Peter Thorpe
CCY232	MATa ADE2 cdc21::kanMX leu2::LEU2-GAL-hENT1 LYS2 RAD5 trp1::TRP1-GAL-dNK ura3-1 RAD52-GFP::HIS3M × 6	This study
CCY234	MATa ADE2 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 RAD52-GFP::HIS3M × 6	This study
CCY118	MATa ADE2 cdc21::natMX leu2::LEU2-GAL-hENT1 LYS2 RAD5 trp1::TRP1-GAL-dNK ura3-1 sgs1∆kanMX	This study
CCY193	MATa ADE2 cdc21::natMX leu2::LEU2-GAL-hENT1 LYS2 RAD5 trp1::TRP1-GAL-dNK ura3-1 rad52∆kanMX	This study
CCY150	MATa ADE2 cdc21::kanMX leu2::LEU2-GAL-hENT1 LYS2 RAD5 trp1::TRP1-GAL-dNK ura3-1 rad51∆natMX	This study
CCY182	MATa ADE2 cdc21::kanMX leu2::LEU2-GAL-hENT1 LYS2 RAD5 trp1::TRP1-GAL-dNK ura3-1 rad52-Y66A	This study
MCY736	MATa/MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 mus81ΔkanMX/MUS81 rad52∆/RAD52 sgs1ΔHIS3/SGS1	This study
MCY737	MATa/MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 mus81ΔkanMX/MUS81 rad51∆natMX/RAD51 sgs1ΔHIS3/SGS1	This study
MCY773	MATa/MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 rad52-Y66A::hphMX/RAD52 sgs1ΔHIS3/SGS1	This study
CCY198	MATa/MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 rad52-Y66A/RAD52 rmi1ΔkanMX/RMI1	This study
MCY735	MATa/MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 mus81ΔkanMX/MUS81 rad52-R70A/RAD52 sgs1ΔHIS3/SGS1	This study
CCY196	MATa/MATα ade2-1/ade2-1 can1-100/can1-100 his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 RAD5/RAD5 rad52-R70A/RAD52 rmi1ΔkanMX/RMI1	This study

elife-31250-v2.xml

10.7554/eLife.31250.024

Table 2.Yeast strains used in this study

Strain	Genotype	Source
H3582	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3392 (sc URA3 TIF11)	(Fekete et al., 2005)
PMY318	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3390 (sc LEU2 TIF11)	This study
PMY284	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5633 (sc LEU2 tif11-K3E)	This study
PMY285	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5635 (sc LEU2 tif11-K4D)	This study
PMY286	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5638 (sc LEU2 tif11-T6D)	This study
PMY287	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5637 (sc LEU2 tif11-T6R)	This study
PMY289	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5640 (sc LEU2 tif11-ΔG8)	This study
PMY290	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5642 (sc LEU2 tif11-R13P)	This study
PMY291	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5644 (sc LEU2 tif11-G15D)	This study
PMY320	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH469 (sc LEU2 tif11-K7A)	This study
PMY321	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH468 (sc LEU2 tif11-K7D)	This study
PMY322	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH481 (sc LEU2 tif11-ΔG8ΔG9)	This study
PMY323	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH471 (sc LEU2 tif11-K10A)	This study
PMY324	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH470 (sc LEU2 tif11-K10D)	This study
PMY325	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH473 (sc LEU2 tif11-R13A)	This study
PMY326	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH472 (sc LEU2 tif11-R13D)	This study
PMY327	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH475 (sc LEU2 tif11-R14A)	This study
PMY329	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH474 (sc LEU2 tif11-R14D)	This study
PMY330	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH478 (sc LEU2 tif11-K16A)	This study
PMY332	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH476 (sc LEU2 tif11-K16D)	This study
PMY354	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3400 (hc LEU2 TIF11)	This study
PMY355	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB167 (hc LEU2 tif11-K4D)	This study
PMY357	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB168 (hc LEU2 tif11-ΔG8)	This study
PMY358	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB169 (hc LEU2 tif11-ΔG8ΔG9)	This study
PMY359	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB170 (hc LEU2 tif11- K10D)	This study
PMY32	MATa ura3-52 leu2-3 leu2-112 trp1Δ−63 his4-301(ACG) sui1Δ::hisG pPMB02 (sc LEU2 sui1-K60E)	(Martin-Marcos et al., 2011)
PMY293	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3390 (sc LEU2 TIF11) p4281 (sc TRP1 TIF5-G31R)	This study
PMY295	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5633 (sc LEU2 tif11-K3E) p4281 (sc TRP1 TIF5-G31R)	This study
PMY296	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5635 (sc LEU2 tif11-K4D) p4281 (sc TRP1 TIF5-G31R)	This study
PMY297	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5638 (sc LEU2 tif11-T6D) p4281 (sc TRP1 TIF5-G31R)	This study
PMY298	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5637 (sc LEU2 tif11-T6R) p4281 (sc TRP1 TIF5-G31R)	This study
PMY300	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5640 (sc LEU2 tif11-ΔG8) p4281 (sc TRP1 TIF5-G31R)	This study
PMY301	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5642 (sc LEU2 tif11-R13P) p4281 (sc TRP1 TIF5-G31R)	This study
PMY302	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5644 (sc LEU2 tif11-G15D) p4281 (sc TRP1 TIF5-G31R)	This study
PMY335	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3390 (sc LEU2 TIF11) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY310	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5633 (sc LEU2 tif11-K3E) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY311	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5635 (sc LEU2 tif11-K4D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY312	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5638 (sc LEU2 tif11-T6D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY313	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5637 (sc LEU2 tif11-T6R) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY315	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5640 (sc LEU2 tif11-ΔG8) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY316	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5642 (sc LEU2 tif11-R13P) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY317	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5644 (sc LEU2 tif11-G15D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY339	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH469 (sc LEU2 tif11-K7A) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY340	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH468 (sc LEU2 tif11-K7D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY341	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH481 (sc LEU2 tif11-ΔG8ΔG9) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY342	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH471 (sc LEU2 tif11-K10A) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY343	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH470 (sc LEU2 tif11-K10D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY344	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH473 (sc LEU2 tif11-R13A) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY345	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH472 (sc LEU2 tif11-R13D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY346	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH475 (sc LEU2 tif11-R14A) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY348	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH474 (sc LEU2 tif11-R14D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY349	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH478 (sc LEU2 tif11-K16A) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY351	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pDH476 (sc LEU2 tif11-K16D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY337	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3390 (sc LEU2 TIF11) YCplac22 (sc TRP1)	This study
PMY338	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3390 (sc LEU2 TIF11) YCplac22 (sc TRP1)	This study
PMY360	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3400 (hc LEU2 TIF11) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY362	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB167 (hc LEU2 tif11-K4D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY364	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB168 (hc LEU2 tif11-ΔG8) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY365	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB169 (hc LEU2 tif11-ΔG8ΔG9) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY366	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ pPMB170 (hc LEU2 tif11- K10D) p4280 (sc TRP1 SUI3-S264Y)	This study
PMY361	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p3400 (hc LEU2 TIF11) YCplac22 (sc TRP1)	This study
GP3511	MATα ura3-52 leu2-3 leu2-112 ino1 sui2∆ gcn2∆ pep4::LEU2 < HIS4 lacZ,ura3−52 > pAV1089 (SUI2,SUI3,GCD11-HIS,URA3)	(Pavitt et al., 1998)
H4560	MATα ura3-52 leu2-3 leu2-112 ino1 sui2Δ gcn2Δ pep4::leu2::natMX sui3Δ::kanMX < HIS4 lacZ,ura3−52 > p5321 (SUI2,SUI3-2,GCD11-HIS,LEU2)	(Martin-Marcos et al., 2014)
YAS2488	MATa leu2-3,−112 his4-53a trp1 ura3-52 cup1::LEU2/PGK1 pG/MFA2 pG	(Algire et al., 2002)
FZY010	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5642 (sc LEU2 tif11-R13P) YCplac22 (sc TRP1)	This study
FZY011	MATa ura3-52 trp1Δ63 leu2-3, leu2-112 his4-301(ACG) tif11Δ p5642 (sc LEU2 tif11-R13P) YCplac22 (sc TRP1)	This study

elife-31981-v2.xml

10.7554/eLife.31981.015

Table 1.Yeast strains used in this study

Strain	Genotype	Reference or source
23344 c	ura3	Laboratory collection
EK008	gap1Δ ura3	Lab collection
ES032	can1Δ ura3	(Gournas et al., 2017)
ES029	gap1Δ can1Δ ura3	(Gournas et al., 2017)
MA032	gap1Δ BUL2-HA ura3	(Merhi and AndreAndré, 2012)
27038a	npi1-1^rsp5 ura3	(Hein et al., 1995)
OS27-1	bul1Δ bul2Δ ura3	Lab collection
34210 c	gap1Δ put4Δ ura3	Lab collection
33007 c	gap1Δ ura3 leu2	Lab collection
30911b	car1 ura3	Lab collection
CG059	gap1Δ can1Δ ura3 leu2	(Gournas et al., 2017)
OS26-1	gtr1Δ gtr2Δ ura3	Lab collection
CEN.PK2-1c	leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8c SUC2 hxt17Δ	(Wieczorke et al., 1999)
EBY.VW4000	hxt1-17Δ gal2Δ stl1Δ agt1Δ mph2Δ mph3Δ leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8c SUC2	(Wieczorke et al., 1999)
I3	EBY.VW4000 URA3::FSY1	(Rodrigues de Sousa et al., 2004)
CG146	seh1Δ ura3	This study
CG148	pib2Δ ura3	This study
CG150	iml1Δ ura3	This study
35652d	fcy1 ura3	This study
ES075	uga1::loxP-kanMX-loxP-GPD1p-pHluorin ura3	This study
YPS14-4	ade 2–101, leu2Δ1, his3-Δ200, ura3–52, trp1Δ63, lys2–801 pma1Δ::HIS3, pma2Δ::TRP1 + YCp-(Sc)PMA1 (LEU2)	(Supply et al., 1993)
PMA4–882Oc hre	ade 2–101, leu2Δ1, his3-Δ200, ura3–52, trp1Δ63, lys2–801 pma1Δ::HIS3, pma2Δ::TRP1 + YEp-PMA1p-(Np)PMA4^882ochre (LEU2)	(Luo et al., 1999)
JW00035	leu2-3-112 ura3-1 trp1-1 his3-11-15 ade2-1 can1-100 sch9Δ::TRP1	(Wilms et al., 2017)
JX023	GAL1p-PMA1 pma2Δ ura3 leu2	This study

elife-32948-v2.xml

10.7554/eLife.32948.022

Table 1.Yeast strains used in this study.

Strain	Genotype
PAS075	Locus2::ade6p::3xE2C:hygMX at Locus2 (between SPBC1711.11 andSPBC1711.12)
PM03	Wild-type strain: h(+); ura4-D18; leu1-32; ade6-M216; his7-366
PM1035	ura4::natMX:dh fragment 1, clr4::KAN as in Marina et al. (2013)
PAS111	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 7 kb, ade6p:3xE2C: hygMX at Locus2
PAS112	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 7 kb, ade6p:3xE2C: hygMX at Locus2; clr4::kanMX
PAS133	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 1 kb, ade6p:3xE2C: hygMX at Locus2; clr4::kanMX
PAS134	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 1 kb, ade6p::3xE2C: hygMX at Locus2
PAS135	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, ade6p::3xE2C: hygMX at Locus2; clr4::kanMX
PAS136	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, ade6p::3xE2C: hygMX at Locus2
PAS141	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 5 kb, ade6p::3xE2C: hygMX at Locus2
PAS142	ura4::natMX:dh:ade6p: SF-GFP, ade6p:mKO2 5 kb; ade6p::3xE2C: hygMX at Locus2; clr4::kanMX
PAS192	ΔK::ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2, h(-)
PAS193	ΔK::ade6p:mKO2; ade6p:SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2; clr4::kanMX, h(-)
PAS214	ΔK::ade6p:mKO2:ura4t; mat3m(EcoRV):: ade6p:SF-GFP; ade6p:3xE2C: hygMX at Locus2; clr4::kanMX, h(-)
PAS215	ΔK::ura4t:mKO2:ade6p; mat3m(EcoRV):: ade6p:SF-GFP; ade6p:3xE2C: hygMX at Locus2; clr4::kanMX, h(-)
PAS216	cenH::ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C: hygMX at Locus2; clr4::kanMX, h90
PAS217	cenH: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C: hygMX at Locus2, h90
PAS218	cenH::ade6p:mKO2 (Kint2); mat3m(EcoRV):: ade6p:SF-GFP; ade6p:3xE2C: hygMX at Locus2; in clr4::kanMX, h90
PAS219	cenH: ade6p:mKO2 (Kint2); mat3m(EcoRV):: ade6p:SF-GFP; ade6p:3xE2C: hygMX at Locus2, h90
PAS231	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, leu1::ade6p:3xE2C: hygMX
PAS237	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, act1p::qxE2C: hygMX at Locus2; clr4::kanMX
PAS243	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, act1p::1xE2C: hygMX at Locus2; clr4::kanMX
PAS244	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, act1p::1xE2C: hygMX at Locus2
PAS264	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C: hygMX at Locus2, pcr1::kanMX, h90
PAS268	ΔK:: ade6p:mKO2; ade6p:SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2, REII::LEU2, h(-)
PAS269	ΔK:: ade6p:mKO2; ade6p:SF-GFP between REIII and mat3M; ade6p:3xE2C:hygMX at Locus2; clr4::kanMX, REII::LEU2, h(-)
PAS331	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C:hygMX at Locus2; ΔREIII::REIII(Δs1, Δs2) in clr4::kanMX, h90
PAS332	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C:hygMX at Locus2; ΔREIII::REIII(Δs1, Δs2), h90
PAS348	ura4::hygMX:REIII:ade6p:SF-GFP; ade6p:mKO2 5 kb, ade6p:3xE2C:natMX at Locus2
PAS350	ura4::hygMX:REIII:ade6p:SF-GFP; ade6p:mKO2 5 kb, ade6p:3xE2C:natMX at Locus2 dcr1::kanMX
PAS355	ura4::natMX:dh:ade6p:SF-GFP, ade6p:mKO2 3 kb, leu1::ade6p:3xE2C:hygMX; clr4::kanMX
PAS385	ΔK:: ade6p:mKO2; ade6p:SF-GFP between REIII and mat3M; act1p:1xE2C:hygMX at Locus2; clr4::kanMX, h(-)
PAS387	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; act1p:1xE2C: hygMX at Locus2, h(-)
PAS388	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ura4 at Locus2; leu1::act1p:1xE2C:hygMX, clr4::kanMX, h90
PAS389	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ura4 at Locus2; leu1::act1p:1xE2C:hygMX, h90
PAS390	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ura4 at Locus2; ΔREIII::REIII(Δs1, Δs2), leu1::act1p:1xE2C:hygMX, in clr4::kanMX, h90
PAS391	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ura4 at Locus2; ΔREIII::REIII(Δs1, Δs2), leu1::act1p:1xE2C:hygMX, h90
PAS398	his1::natMX:dh:ade6p:mKO2; ade6p:SF-GFP 3 kb, ade6p::3xE2C:hygMX at Locus2, clr4::kanMX, ura4::phyB.
PAS399	his1::natMX:dh:ade6p:mKO2; ade6p:SF-GFP 3 kb, ade6p::3xE2C:hygMX at Locus2, ura4::phyB.
PAS410	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p::3xE2C:hygMX at Locus2, natMX:clr4+, h(-); ‘OFF’ allele
PAS411	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p::3xE2C:hygMX at Locus2, natMX:clr4+, h(-); ‘ON’ allele
PAS464	ΔK::ade6p:mKO2:ura4t; mat3m(EcoRV):: ade6p:SF-GFP; ade6p:3xE2C: hygMX at Locus2; natMX:clr4+, h(-)
PAS465	ΔK::ura4t:mKO2:ade6p; mat3m(EcoRV):: ade6p:SF-GFP; ade6p:3xE2C: hygMX at Locus2; natMX:clr4+, h(-)
PAS473	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; 700 bp sib1 ORF between REIII-s1 and mKO2; ade6p:3xE2C: hygMX at Locus2, clr4::kanMX, h(-);
PAS474	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ΔREIII::REIII(Δs1, Δs2), ade6p:3xE2C: hygMX at Locus2, clr4::kanMX, h(-);
PAS478	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; 700 bp sib1 ORF between REIII-s1 and mKO2 ade6p:3xE2C: hygMX, natMX:clr4+, h(-);
PAS482	ΔK::ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2, h(-); ‘OFF’ allele
PAS483	ΔK:: ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ΔREIII::REIII(Δs1, Δs2), ade6p:3xE2C: hygMX at Locus2, natMX:clr4+, h(-);
PAS496	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C:hygMX at Locus2; ΔREIII::REIII(Δs1, Δs2), ars1::prad15:cre-EBD:LEU2; h3.2:lox:HA:hygMX:lox:T7; h90
PAS497	ΔK::ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2; ars1::prad15:cre-EBD:LEU2; h3.2:lox:HA:hygMX:lox:T7; ‘OFF’ allele, h(-)
PAS498	ΔK::ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2; ars1::prad15:cre-EBD:LEU2; h3.2:lox:HA:hygMX:lox:T7; ‘ON’ allele; h(-)
PAS508	ΔK::ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2, ‘OFF’ allele; pcr1::kanMX
PAS510	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C:hygMX at Locus2; ΔREIII::REIII(Δs1, Δs2), pcr1::kanMX
PAS514	ΔK::ade6p:mKO2; ade6p: SF-GFP between REIII and mat3M; ade6p:3xE2C: hygMX at Locus2, ‘OFF’ allele; dcr1::kanMX; seb1-1:natMX
PAS515	cenH:: ade6p:SF-GFP (Kint2); mat3m(EcoRV):: ade6p:mKO2; ade6p:3xE2C:hygMX at Locus2; ΔREIII::REIII(Δs1, Δs2), seb1-1:natMX

elife-35388-v1.xml

10.7554/eLife.35388.013

Table 1.Yeast strains used in this study.

All strains are derived from WL001 and WL002. All Ire1 constructs listed below are 3x FLAG-tagged within their lumenal domains.

Strain	Species	Description
yWL001	Sc	WT, mat A, leu2-3,112 TRP1 can1-100 ura3-1 ADE2 his3-11,15
yWL002	Sc	ire1∆::NAT^R
yWL003	Sc	ire1∆::NAT^R, HO::Sp IRE1
yWL004	Sc	ire1∆::NAT^R, HO::SpLumScCyto IRE1
yWL005	Sc	ire1∆::NAT^R, HO::ScLumSpCyto IRE1
yWL006	Sc	ire1∆::NAT^R, HO::Sc IRE1
yWL007	Sc	ire1∆::NAT^R, HO::ScLum/transmembrane/linkerSpKR IRE1
yWL008	Sc	WT, leu2::5'hac1-gfp-3'hac1
yWL009	Sc	ire1∆::NAT^R, leu2::5'hac1-gfp-3'hac1
yWL010	Sc	ire1∆::NAT^R, leu2::5'hac1-gfp-3'hac1, HO::Sp IRE1
yWL011	Sc	ire1∆::NAT^R, leu2::5'hac1-gfp-3'hac1, HO:: SpLumScCyto IRE1
yWL012	Sc	ire1∆::NAT^R, leu2::5'hac1-gfp-3'hac1, HO:: ScLumSpCyto IRE1
yWL013	Sc	ire1∆::NAT^R, leu2::5'hac1-gfp-3'hac1, HO::Sc IRE1
yWL014	Sc	leu2::5'hac1-gfp-3'hac1, his3::pTdh3-mCherry
yWL015	Sp	WT, mat h⁺, ade6-M210, ura4-D18, leu1-32
yWL016	Sp	ire1∆::KAN^R
yWL017	Sp	ire1∆::KAN^R, leu1::Sp IRE1
yWL018	Sp	ire1∆::KAN^R, leu1::SpLumScCyto IRE1
yWL019	Sp	ire1∆::KAN^R, leu1::ScLumSpCyto IRE1
yWL020	Sp	ire1∆::KAN^R, leu1::Sc IRE1
yWL021	Sp	ire1∆::KAN^R, leu1::SpLumScCyto IRE1, ura4::5'hac1-gfp-3'hac1
yWL022	Sp	ire1∆::KAN^R, leu1::ScLumSpCyto IRE1, ura4::5'hac1-gfp-3'hac1
yWL023	Sp	ire1∆::KAN^R, leu1::Sc IRE1, ura4::5'hac1-gfp-3'hac1
yWL024	Sp	bip1::bip1-hac1 hybrid
yWL025	Sp	bip1::bip1-hac1 hybrid A->U
yWL026	Sp	bip1::bip1 splicing variant
yWL027	Sp	ura4::synthetic splicing substrate

elife-43088-v2.xml

10.7554/eLife.43088.010

Table 1.Yeast strains used in this study.

Name	Genotype	Figures	Reference
W303-1a	MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100	-	(Thomas and Rothstein, 1989)
ScKH146	W303-1A, ade2::ADE2 ATG5-EGFP-kanMX6 ATG17-2×mCherry-hphNT1	1A	This study
ScKH153	ScKH146 atg16Δ::natNT2	1A	This study
ScKH182	ScKH146 atg21Δ::zeoNT3	1A	This study
ScKH151	ScKH146 atg14Δ::natNT2	1A	This study
ScKH149	ScKH146 atg12Δ::natNT2	1A	This study
ScKH162	ScKH146 atg14Δ::natNT2 atg12Δ::zeoNT3	1A	This study
ScTK623	ScKH146 atg21Δ::natNT2 atg12Δ::zeoNT3	1A	This study
BJ2168	MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2	2A-C, 2E, 2F, 2-S1A, 2-S1C	(Jones, 1991)
MAN169	BJ2168 ATG5-TEV-3×FLAG-kanMX4	2A, 2C, 2E, 2F, 2-S1A, 2-S1C	This study
ScKH10	MAN169 atg16Δ::natNT2	2A, 2E, 2-S1A	This study
ScKH32	MAN169 atg12Δ::natNT2	2A, 3A	This study
ScKH96	MAN169 atg10Δ::natNT2	2A	This study
ScKH90	BJ2168 ATG16-TEV-3×FLAG-kanMX4	2B	This study
ScKH92	ScKH90 atg5Δ::natNT2	2B	This study
ScKH93	ScKH90 atg12Δ::natNT2	2B	This study
ScKH141	MAN169 atg1Δ::natNT2	2C	This study
ScKH99	MAN169 atg13Δ::natNT2	2C, 2D	This study
ScKH216	MAN169 atg17Δ::natNT2	2C	This study
ScKH101	MAN169 atg29Δ::natNT2	2C	This study
ScKH143	MAN169 atg31Δ::natNT2	2C	This study
ScKH98	MAN169 atg14Δ::natNT2	2E	This study
ScKH97	MAN169 atg11Δ::natNT2	2F	This study
ScYH3184	BJ2168 leu2::LEU2	2G	This study
ScKH66	ScHY3184 ATG5-TEV-3×FLAG-kanMX4	2G	This study
ScKH68	ScHY3184 atg1^D211A-hphNT1 ATG5-TEV-3×FLAG-kanMX4	2G	This study
AH109	MATa trp1-901 leu2-3, 112 ura3-52 his3-200 gal4Δ gal80ΔLYS2::GAL1_UAS-GAL1_TATA-HIS3 MEL1 GAL2_UAS-GAL2_TATA-ADE2 URA3::MEL1_UAS-MEL1_TATA-lacZ	2-S2A	Clontech
ScTK967	AH109 atg13Δ::natNT2 atg31Δ::hphNT1	2-S2B	This study
ScTK968	AH109 atg17Δ::natNT2	2-S2C	This study
ScTK877	BJ2168 atg11Δ::LEU2 atg29Δ::zeoNT3 atg31Δ::hphNT1 atg1Δ::natNT2 atg13Δ::Klura3 P_ADH1-ATG17-CgTRP1	2-S2D	This study
ScTK958	ScTK877 KlURA3-P_ADH1-ATG12-EGFP-kanMX4	2-S2D	This study
ScTK649	ScTK623 his3-11::pRS303	3B	This study
ScTK650	ScTK623 his3-11::pRS303-ATG12	3B	This study
ScTK651	ScTK623 his3-11::pRS303-atg12^Δ^N56	3B	This study
BY4741	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0	-	(Brachmann et al., 1998)
ScTK557	BY4741 pho8Δ::kanMX4- P_GPD-pho8Δ60 atg12Δ::natNT2	3C, 4A	This study
ScTK559	BY4741 pho8Δ::kanMX4- P_GPD-pho8Δ60 atg21Δ::zeoNT3 atg12Δ::natNT2	3C, 4A	This study
ScKH119	W303-1A, ade2Δ::ADE2 ATG17-EGFP-kanMX4 atg11Δ::zeoNT3	4B	This study
ScKH121	ScKH119 atg5Δ::natNT2	4B	This study
ScKH123	ScKH119 atg12Δ::natNT2	4B	This study
ScKH125	ScKH119 atg16Δ::natNT2	4B	This study
ScTK657	YKH123 his3-11::pRS303	4C	This study
ScTK658	YKH123 his3-11::pRS303-ATG12	4C	This study
ScTK659	YKH123 his3-11::pRS303-atg12^Δ^N56	4C	This study

elife-48215-v2.xml

10.7554/eLife.48215.012

Table 2.Yeast strains used in this study.

Strain number	Genotype	Reference
SMH81	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX	(Huh et al., 2003)
SMH91	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX iml3Δ::KanMX	This study
SMH690	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX CTF3-3FLAG::KanMX	This study
SMH691	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX CTF3-3FLAG::KanMX	This study
SMH692	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX CTF3-3FLAG::KanMX	This study
SMH693	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX ctf3-SDD-3FLAG::KanMX	This study
SMH694	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX ctf3-SDD-3FLAG::KanMX	This study
SMH695	MATa MCM22-GFP::HisMX SPC110-mCherry::hphMX ctf3-SDD-3FLAG::KanMX	This study

elife-58243-v2.xml

Table 3.Yeast strains used in this study.

Strain	Description	Source
F2041/yRP2799	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pRP1560 (DED1 URA3)	Hilliker et al., 2011
H3666	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 DED1-myc₁₃ HIS3	Dong et al., 2005
F729/BY4741	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0	Research Genetics
H4436^*	MATa ade2 his3 leu2 trp1 ura3 pep4::HIS3 tif4631::leu2hisG tif4632::ura3 pEP88 (TIF4631-HA-Bam TRP1 CEN4)	Park et al., 2011
F694	MATa cdc33::LEU2 ade2 his3 leu2 trp1 ura3 p(CDC33, TRP1, ARS/CEN4)	Altmann and Trachsel, 1989
F695	MATa cdc33::LEU2 ade2 his3 leu2 trp1 ura3 p(cdc33-4-2, TRP1, ARS/CEN4)	Altmann and Trachsel, 1989
F696	MATa cdc33::LEU2 ade2 his3 leu2 trp1 ura3 p(cdc33-1, TRP1, ARS/CEN4)	Altmann and Trachsel, 1989
SGY1	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG1 (DED1-myc LEU2)	This study
SGY2	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG2 (ded1-ts-myc LEU2)	This study
SGY3	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG3 (ded1-ts/4–10-myc LEU2)	This study
SGY4	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG4 (ded1-ts/14–20-myc LEU2)	This study
SGY5	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG5 (ded1-ts/21–27-myc LEU2)	This study
SGY6	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG6 (ded1-ts/29–35-myc LEU2)	This study
SGY7	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG7 (ded1-ts/Δ40–47-myc LEU2)	This study
SGY8	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG8 (ded1-ts/51–57-myc LEU2)	This study
SGY9	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG9 (ded1-ts/59–65-myc LEU2)	This study
SGY10	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG10 (ded1-ts/68–74-myc LEU2)	This study
SGY11	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG11 (ded1-ts/83–89-myc LEU2)	This study
SGY12	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG12 (ded1-ts/90–95-myc LEU2)	This study
SGY13	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG13 (ded1-ts/21-27/29-35-myc LEU2)	This study
SGY14	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG14 (ded1-ts/21-27/51-57-myc LEU2)	This study
SGY15	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG15 (ded1-ts/59-65/83-89-myc LEU2)	This study
SGY16	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG16 (ded1-ts/21-27/51-57/59-65/83-89-myc LEU2)	This study
SGY17	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG17 (ded1-ts,Δ2–90-myc LEU2)	This study
SGY18	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG18 (ded1-ts,Δ562–604-myc LEU2)	This study
SGY19	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG19 (ded1-ts/21-27/51-57/59-65/83-89-myc/Δ562–604-myc LEU2)	This study
SGY20	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG20 (ded1-ts,Δ2–90,Δ562–604-myc LEU2)	This study
SGY21	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG21 (ded1-ts/Y21A-myc LEU2)	This study
SGY22	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG22 (ded1-ts/R27A-myc LEU2)	This study
SGY23	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG23 (ded1-ts/G28A-myc LEU2	This study
SGY24	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG24 (ded1-ts/F56A/F57A-myc LEU2)	This study
SGY25	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG25 (ded1-ts/Y65A-myc LEU2)	This study
SGY26	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG26 (ded1-ts/W88A-myc LEU2)	This study
SGY27	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG27 (ded1-21-27-myc LEU2)	This study
SGY28	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG28 (ded1-51-57-myc LEU2)	This study
SGY29	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG29 (ded1-59-65-myc LEU2)	This study
SGY30	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG30 (ded1-83-89-myc LEU2)	This study
SGY31	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG31 (ded1-21-27,51–57-myc LEU2)	This study
SGY32	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG32 (ded1-59-65,83–89-myc LEU2)	This study
SGY33	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG33 (ded1-21-27/51-57/59-65/83-89-myc-myc LEU2)	This study
SGY34	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG34 (ded1Δ2–90-myc LEU2)	This study
SGY35	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG35 (ded1Δ562–604-myc LEU2)	This study
SGY36	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG36 (ded1-21-27/Δ562–604-myc LEU2)	This study
SGY37	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG37 (ded1-51-57/Δ562–604-myc LEU2)	This study
SGY38	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG38 (ded1-59-65/Δ562–604-myc LEU2)	This study
SGY39	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG39 (ded1-83-89/Δ562–604-myc LEU2)	This study
SGY40	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG40 (ded1-21-27/51-57/Δ562–604-myc LEU2)	This study
SGY41	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG41 (ded1-59-65/83-89/Δ562–604-myc LEU2)	This study
SGY42	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG42 (ded1-21-27/51-57/59-65/83-89-myc/Δ562–604-myc LEU2)	This study
SGY43	MATa his3Δ1 leu2Δ0 lys2Δ0 met15Δ0 ura3Δ0 ded1Δ::kanMX4 pSG43 (ded1-Δ2–90,Δ562–604-myc LEU2)	This study

*The plasmid in this strain contains TIF4631-HA under the control of the TIF4632 promoter and transcription terminator, as described previously (Tarun and Sachs, 1996) modified to insert a BamHI site at the start codon (Park et al., 2011).